Interactions of Amino Group Functionalized Tetraphenylvinyl and DNA: A Label-Free “On-Off-On” Fluorescent Aptamer Sensor toward Ampicillin
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Published:2023-04-27
Issue:5
Volume:13
Page:504
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ISSN:2079-6374
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Container-title:Biosensors
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language:en
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Short-container-title:Biosensors
Author:
Geng Weifu1, Feng Yan2, Chen Yu2, Zhang Xin1, Zhang Haoyi2, Yang Fanfan2, Wang Xiuzhong12ORCID
Affiliation:
1. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China 2. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
Abstract
As a type of aggregation-induced emission (AIE) fluorescent probe, tetraphenylvinyl (TPE) or its derivatives are widely used in chemical imaging, biosensing and medical diagnosis. However, most studies have focused on molecular modification and functionalization of AIE to enhance the fluorescence emission intensity. There are few studies on the interaction between aggregation-induced emission luminogens (AIEgens) and nucleic acids, which was investigated in this paper. Experimental results showed the formation of a complex of AIE/DNA, leading to the quenching of the fluorescence of AIE molecules. Fluorescent test experiments with different temperatures proved that the quenching type was static quenching. The quenching constants, binding constants and thermodynamic parameters demonstrated that electrostatic and hydrophobic interactions promoted the binding process. Then, a label-free “on-off-on” fluorescent aptamer sensor for the detection of ampicillin (AMP) was constructed based on the interaction between the AIE probe and the aptamer of AMP. Linear range of the sensor is 0.2–10 nM with a limit of detection 0.06 nM. This fluorescent sensor was applied to detect AMP in real samples.
Funder
the National Innovation Training Program for College Students Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
Reference37 articles.
1. Luo, J., Xie, Z., Lam, J.W.Y., Cheng, L., Chen, H., Qiu, C., Kwok, H.S., Zhan, X., Liu, Y., and Zhu, D. (2001). Aggregation-induced emission of 1-methyl-1,2,3,4,5-pentaphenylsilole. Chem. Commun., 1740–1741. 2. ACQ-to-AIE Transformation: Tuning Molecular Packing by Regioisomerization for Two-Photon NIR Bioimaging;Li;Angew. Chem. Int. Ed.,2020 3. Kaempferol as an AIE-active natural product probe for selective Al3+ detection in Arabidopsis thaliana;Sun;Spectrochim. Acta A Mol. Biomol. Spectrosc.,2021 4. Aggregation-Induced Emission: Recent Advances in Materials and Biomedical Applications;Cai;Angew. Chem. Int. Ed.,2020 5. Biosensing by luminogens with aggregation-induced emission characteristics;Kwok;Chem. Soc. Rev.,2015
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