Using Sparfloxacin-Capped Gold Nanoparticles to Modify a Screen-Printed Carbon Electrode Sensor for Ethanol Determination
Author:
Magesh Vasanth1ORCID, Kothari Vishaka S.1, Ganapathy Dhanraj1, Atchudan Raji2ORCID, Arya Sandeep3ORCID, Nallaswamy Deepak1, Sundramoorthy Ashok K.1ORCID
Affiliation:
1. Centre for Nano-Biosensors, Department of Prosthodontics, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals, 162 Poonamallee High Road, Velappanchavadi, Chennai 600077, India 2. School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea 3. Department of Physics, University of Jammu, Jammu 180006, India
Abstract
Alcohol is a dangerous substance causing global mortality and health issues, including mental health problems. Regular alcohol consumption can lead to depression, anxiety, cognitive decline, and increased risk of alcohol-related disorders. Thus, monitoring ethanol levels in biological samples could contribute to maintaining good health. Herein, we developed an electrochemical sensor for the determination of ethanol in human salivary samples. Initially, the tetra-chloroauric acid (HAuCl4) was chemically reduced using sparfloxacin (Sp) which also served as a stabilizing agent for the gold nanoparticles (AuNPs). As-prepared Sp-AuNPs were comprehensively characterized and confirmed by UV-visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and elemental mapping analysis. The average particle size (~25 nm) and surface charge (negative) of Sp-AuNPs were determined by using dynamic light scattering (DLS) and Zeta potential measurements. An activated screen-printed carbon electrode (A-SPE) was modified using Sp-AuNPs dispersion, which exhibited greater electrocatalytic activity and sensitivity for ethanol (EtOH) oxidation in 0.1 M sodium hydroxide (NaOH) as studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). DPV showed a linear response for EtOH from 25 µM to 350 µM with the lowest limit of detection (LOD) of 0.55 µM. Reproducibility and repeatability studies revealed that the Sp-AuNPs/A-SPEs were highly stable and very sensitive to EtOH detection. Additionally, the successful electrochemical determination of EtOH in a saliva sample was carried out. The recovery rate of EtOH spiked in the saliva sample was found to be 99.6%. Thus, the incorporation of Sp-AuNPs within sensors could provide new possibilities in the development of ethanol sensors with an improved level of precision and accuracy.
Funder
Science and Engineering Research Board (SERB), India Department of Science and Technology, India
Subject
Electrical and Electronic Engineering,Biochemistry,Instrumentation,Atomic and Molecular Physics, and Optics,Analytical Chemistry
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