Affiliation:
1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
2. Institute of Specialty Crop, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China
3. Chongqing Three Gorges Academy of Agricultural Sciences, Chongqing 404100, China
Abstract
Soybeans are the main source of vegetable protein and edible oil for humans, with an average content of about 40% crude protein and 20% crude fat. Soybean quality traits are mostly quantitative traits controlled by multiple genes. The quantitative trait loci (QTL) for soybean quality traits and mining related candidate genes are of great significance for the molecular breeding of soybean quality traits and understanding the genetic mechanism of protein/fat metabolism. In this study, the F2 population was derived from the high-protein material Changjiang Chun 2 and Jiyu 166. On the basis of a genetic linkage map constructed in our previous study, the QTL of crude protein content, crude oil content and fatty acid fractions were detected using the multiple-QTL model (MQM) mapping method. The results show that a total of 92 QTL were obtained affecting quality traits under three environments, including 14 QTL of crude oil content, 9 QTL of crude protein content, and 20, 20, 11, 10 and 8 QTL for the content of palmitic, stearic, oleic, linoleic and linolenic acids, respectively. Sixteen QTL clusters were identified, among which Loci01.1, Loci06.1 and Loci11.1 were identified as stable QTL clusters with phenotypic contribution rates of 16.5%, 16.4% and 12.1%, respectively, and candidate genes were mined in their regions. A total of 32 candidate genes related to soybean quality were finally screened via GO enrichment and gene annotation. The present study lies the foundations for understanding the genetic mechanism and elite germplasm innovation of seed quality in soybean.
Funder
Chongqing Technology Innovation and Application Development Special Key Project
Collection, Utilization and Innovation of Germplasm Resources by Research Institutes and Enterprises of Chongqing
Subject
Agronomy and Crop Science
Reference38 articles.
1. Genomics progress will facilitate molecular breeding in soybean;Wang;Sci. China Life Sci.,2015
2. Establishment of a Predictive Model to Identify Suitable Soybean Genotypes for Oil Processing;Sun;J. Food Compos. Anal.,2022
3. Kim, I.-S., Kim, C.-H., and Yang, W.-S. (2021). Physiologically Active Molecules and Functional Properties of Soybeans in Human Health—A Current Perspective. Int. J. Mol. Sci., 22.
4. Functional and edible uses of soy protein products;Singh;Compr. Rev. Food Sci. Food Saf.,2008
5. Endocrinological response to soy protein and fiber in mildly hypercholesterolemic men;Ham;Nutr. Res.,1993
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献