Responses of Physiological Traits and Grain Yield to Short Heat Stress during Different Grain-Filling Stages in Summer Maize

Abstract

Maize kernel growth is sensitive to heat stress, which is predicted to result in yield loss. However, the response of maize to short-term heat stress during different kernel-growth stages is still not clear. A 3-year field experiment, included two heat-stress treatments (LSH, lag-stage heat stress; FSH, effective-filling-stage heat stress), was conducted in 2019–2021. The results showed that LSH and FSH significantly reduced the grain yield by 8.7–14.9% and 11.6–17.6%, respectively, compared with the control (CK). LSH mainly reduced the kernel number per ear, and FSH mainly reduced the kernel weight. Heat stress reduced the SPAD and chlorophyll content during the effective-filling stage, but not in the lag stage. Photosynthesis was obviously reduced under heat stress during both stages; however, the photosynthesis rate during the lag stage was higher than during the effective-filling stage. The decreased amplitude of dry matter under LSH was higher than under FSH during the heating period. However, there was no difference between the CK and LSH samples in terms of dry-matter accumulation from the end of heat stress to harvest, which was significantly reduced under FSH. Additionally, decreased starch-synthesis-related enzyme activity, i.e., adenosine diphos-phoglucose pyrophosphprylase, contributed to the kernel number and kernel weight loss in LSH and FSH, respectively. The kernel-growth rate (per ear) was lowered, but the growth duration was not shortened in this study. Consequently, LSH mainly reduced the photosynthesis rate (Pn), resulting in kernel abortion and yield penalty. FSH mainly reduced the Pn and chlorophyll content, which reduced the kernel weight, and increased yield loss.