Comparative Transcriptome Analysis and Expression of Genes Associated with Polysaccharide Biosynthesis in Dendrobium officinale Diploid and Tetraploid Plants
Author:
Pham Phu Long1, Le Thi Tuyet Cham1, Vu Thi Thuy Hang1, Nguyen Thanh Tuan1, Zhang Zhi-Sheng2, Zeng Rui-Zhen2, Xie Li2, Nguyen Minh Ngoc3, Trang Vuong Thi Huyen3, Xuan Tran Dang4ORCID, Dang Khanh Tran3ORCID
Affiliation:
1. Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi City 131000, Vietnam 2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China 3. Department Genetic Engineering, Agricultural Genetics Institute, Pham Van Dong Street, Hanoi City 122000, Vietnam 4. Department of Development Technology, Graduate School for International Development and Cooperation, Hiroshima University, Hiroshima 739-8529, Japan
Abstract
Dendrobium officinale Kimura et Migo is a kind of herb with high medicinal, ornamental, and commercial value, and is rich in polysaccharides. Polyploid breeding is an important breeding method for the genome doubling of medicinal species to increase biomass and polysaccharide production. Previous studies have revealed comparative transcriptome analysis and polysaccharide biosynthesis across the growth stages and plant parts, but there have been no studies dissecting such genes and pathways in tetraploid D. officinale. Therefore, this study aimed to unravel the molecular mechanisms of the increase in polysaccharide content in tetraploid D. officinale via the generation of four transcriptomic libraries for protocorm-like bodies and six-month-old seedlings of both diploid and tetraploid D. officinale plants. In this study, a total of 230,786,618 clean reads remained with a total of 34.62 Gb nucleotides generated; 274,403 unigenes were assembled, of which 73.99% were annotated to at least one of the protein databases; and of 17,451 unigenes, 6.35% were annotated to all seven protein databases (NR, NT, KO, Swiss-Prot, FAM, GO, and KOG). Putative genes encoding enzymes related to polysaccharide biosynthetic pathways were determined. RT-qPCR for 11 randomly selected genes involved in polysaccharides indicated consistency with RNA-Seq data and polysaccharide content. The expressions of nine genes were higher in tetraploid than in diploid plants, while the expressions of the other two genes encoding bifunctional enzymes were the opposite. This study has provided a foundation for subsequent works regarding the biosynthetic pathways of metabolites involved in the autoploidy of Dendrobium species in general, and D. officinale in particular.
Funder
Vietnam National University of Agriculture
Subject
Agronomy and Crop Science
Reference67 articles.
1. Polysaccharides; classification, chemical properties, and future perspective applications in fields of pharmacology and biological medicine (A review of current applications and upcoming potentialities);Mohammed;J. Polym. Environ.,2021 2. Claus-Desbonnet, H., Nikly, E., Nalbantova, V., Karcheva-Bahchevanska, D., Ivanova, S., Pierre, G., Benbassat, N., Katsarov, P., Michaud, P., and Lukova, P. (2022). Polysaccharides and their derivatives as potential antiviral molecules. Viruses, 14. 3. Polysaccharide immunomodulators as therapeutic agents: Structural aspects and biological function;Tzianabos;Clin. Microbiol. Rev.,2000 4. Purification, characterization, and anticoagulant activity of the polysaccharides from green tea;Cai;Carbohydr. Polym.,2013 5. Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.);Lee;Int. Immunopharmacol.,2013
|
|