Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery: Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation-Reference-Cited by-同舟云学术

Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery: Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation

Published:2023-05-15 Issue:10 Volume:24 Page:8776
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Ruiz Victor H.¹^ORCID,Encinas-Basurto David¹²^ORCID,Sun Bo¹³,Eedara Basanth Babu¹⁴^ORCID,Roh Eunmiri⁵⁶^ORCID,Alarcon Neftali Ortega¹,Curiel-Lewandrowski Clara³⁷⁸^ORCID,Bode Ann M.⁵,Mansour Heidi M.¹⁴⁹¹⁰^ORCID

Affiliation:

1. Skaggs Pharmaceutical Sciences Center, College of Pharmacy, The University of Arizona, Tucson, AZ 85721, USA

2. Department of Physics, Mathematics and Engineering, Campus Navojoa, Universidad de Sonora, Sonora 85880, Mexico

3. The University of Arizona Cancer Center, Skin Cancer Institute, Tucson, AZ 85721, USA

4. Center for Translational Science, Florida International University, Port St. Lucie, FL 34987, USA

5. The Hormel Institute, University of Minnesota, Austin, MN 55912, USA

6. Department of Cosmetic Science, Kwangju Women’s University, Gwangju 62396, Republic of Korea

7. University of Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, USA

8. Department of Medicine, Division of Dermatology, The University of Arizona College of Medicine, Tucson, AZ 85724, USA

9. Department of Medicine, Division of Translational & Regenerative Medicine, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA

10. BIO5 Institute, The University of Arizona, Tucson, AZ 85721, USA

Abstract

Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the development of UV-induced cSCC. This study aimed to investigate the physicochemical properties and in vitro behavior of RocBr. Techniques such as thermal analysis, electron microscopy, spectroscopy and in vitro assays were used to characterize RocBr. A topical oil/water emulsion lotion formulation of RocBr was successfully developed and evaluated. The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M® synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. Significant membrane retention of RocBr drug was evident and more retention was obtained with the lotion formulation compared with the solution. This is the first systematic and comprehensive study to report these findings.

Funder

NIH NCI Project

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Link

https://www.mdpi.com/1422-0067/24/10/8776/pdf

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