In Vitro Fermentation of Polysaccharide from Edible Alga Enteromorpha clathrata by the Gut Microbiota of Patients with Ulcerative Colitis
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Published:2023-09-24
Issue:19
Volume:15
Page:4122
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Ma Mingfeng12, Quan Min1, Zhang Jiaxue1, Zhang Aijun3, Gao Puyue3, Shang Qingsen14, Yu Guangli12ORCID
Affiliation:
1. Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China 2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China 3. Qilu Hospital of Shandong University (Qingdao), Qingdao 266035, China 4. Qingdao Marine Biomedical Research Institute, Qingdao 266071, China
Abstract
Dietary intake of the sulfated polysaccharide from edible alga E. clathrata (ECP) has recently been illustrated to attenuate ulcerative colitis (UC) by targeting gut dysbiosis in mice. However, ECP is not easily absorbed in the gut and, as a potential candidate for next-generation prebiotics development, how it is fermented by human gut microbiota has not been characterized. Here, using in vitro anaerobic fermentation and 16S high-throughput sequencing, we illustrate for the first time the detailed fermentation characteristics of ECP by the gut microbiota of nine UC patients. Our results indicated that, compared to that of glucose, fermentation of ECP by human gut microbiota produced a higher amount of anti-inflammatory acetate and a lower amount of pro-inflammatory lactate. Additionally, ECP fermentation helped to shape a more balanced microbiota composition with increased species richness and diversity. Moreover, ECP significantly stimulated the growth of anti-colitis bacteria in the human gut, including Bacteroides thetaiotaomicron, Bacteroides ovatus, Blautia spp., Bacteroides uniformis, and Parabacteroides spp. Altogether, our study provides the first evidence for the prebiotic effect of ECP on human gut microbiota and sheds new light on the development of ECP as a novel prebiotic candidate for the prevention and potential treatment of UC.
Funder
National Natural Science Foundation of China Natural Science Foundation of Shandong Province Fundamental Research Funds for the Central Universities Taishan Scholars Program Shandong Provincial Major Science and Technology Project Major Project of Qingdao National Laboratory for Marine Science and Technology Taishan Industry Leading Talent Project, and Taishan Scholar Climbing Project
Subject
Food Science,Nutrition and Dietetics
Reference40 articles.
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