Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
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Published:2023-03-24
Issue:4
Volume:45
Page:2703-2716
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ISSN:1467-3045
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Container-title:Current Issues in Molecular Biology
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language:en
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Short-container-title:CIMB
Author:
Zhang Chuanxi1,
Kang Yumeng1,
Zhang Ziyan1,
Liu Heliang2,
Xu Hong2ORCID,
Cai Wenchen2,
Gao Xuemin2,
Yang Jie1
Affiliation:
1. Clinical Medical College, North China University of Science and Technology, Tangshan 063210, China
2. School of Public Health, North China University of Science and Technology, Tangshan 063210, China
Abstract
Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson’s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration.
Funder
National Natural Science Foundation of China
National Natural Science Foundation of Hebei Province
Science and Technology Project of Hebei Education Department
Technology Project of Tangshan
Non-profit Central Research Institute Fund of Chinese Academy of Medical Science
Project of Key Laboratory of Functional and Clinical Translational Medicine
Subject
Microbiology (medical),Molecular Biology,General Medicine,Microbiology
Cited by
1 articles.
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