Chloride Intracellular Channel Protein 1 Expression and Angiogenic Profile of Liver Metastasis of Digestive Origin
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Published:2023-02-06
Issue:2
Volume:45
Page:1396-1406
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ISSN:1467-3045
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Container-title:Current Issues in Molecular Biology
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language:en
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Short-container-title:CIMB
Author:
Ceausu Amalia Raluca1ORCID, Ciolofan Alexandru2, Blidisel Alexandru2ORCID, Cosma Andrei Alexandru1, Gaje Pusa Nela1, Cretu Octavian2
Affiliation:
1. Angiogenesis Research Center, Department of Microscopic Morphology/Histology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Sq. Eftimie Murgu No. 2, 300041 Timisoara, Timis, Romania 2. Department of Surgical Semiology, “Victor Babes” University of Medicine and Pharmacy, Sq. Eftimie Murgu No. 2, 300041 Timisoara, Timis, Romania
Abstract
Chloride intracellular channel 1 (CLIC1) is involved in cell migration and metastasis. The histological growth patterns of liver metastasis are as follows: desmoplastic (d-HGP), replacement (r-HGP), pushing (p-HGP), and mixed. The aim of this study was to evaluate the relation between HGP, angiogenesis, and CLIC1 expression. Materials and Methods: A total of 40 cases of primary tumors and their LM: d-HGP (12 cases), r-HGP (13 cases), and p-HGP (15 cases), were evaluated through simple and double immunostaining. CLIC1 assessment was conducted as follows: scores of 0 (less than 10% of positive cells), 1 (10–30%), 2 (30–50%), or 3 (more than 50%) were assigned. Heterogeneous CLIC1 expression was found. CLIC1 in primary tumors correlated with grade G for all cases of LM with a p-HGP (p = 0.004). The CLIC1 score for LMs with an r-HGP correlated with grade G of the corresponding primary tumor (p = 0.027). CLIC1 and CD34+/Ki67+ vessels (p = 0.006) correlated in primary tumors. CLIC1 in primary tumors correlated with CD34+/Ki67+ vessels of LMs with a d HGP (p = 0.024). Conclusions: The CLIC1 score may have prognostic value, mainly for LMs with a p-HGP and r-HGP, and therapeutic value for LMs with a d-HGP.
Funder
“Victor Babes” University of Medicine and Pharmacy Angiogenesis Research Center
Subject
Microbiology (medical),Molecular Biology,General Medicine,Microbiology
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