Morphometric Characteristics and Genetic Issr Marker Variability in Rhodiola rosea L. (Crassulaceae) in Different Ecological and Geographic Conditions in the Altai Republic
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Published:2023-10-16
Issue:20
Volume:24
Page:15224
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Dorogina Olga V.1, Kuban Irina N.1, Achimova Altynai A.2, Williams Natasha3, Lashchinskiy Nicolay N.14, Zhmud Elena V.1
Affiliation:
1. Central Siberian Botanical Garden SB RAS, Zolotodolinskaya Str. 101, Novosibirsk 630090, Russia 2. Gorno-Altai Botanical Garden, Chisty Lug, Shebalinsky District, Kamlak 649218, Russia 3. Department of Food Science and Human Nutrition, Colorado State University, 502 W Lake St., Fort Collins, CO 80523, USA 4. NSU Climate Center, Novosibirsk State University, Pirogova Str. 1, Novosibirsk 630090, Russia
Abstract
Rhodiola rosea L. is a vulnerable species in the Altai Republic (AR) and Russia in general. For the first time on the territory of AR, studies of the adaptive capabilities of the species and genetic differentiation using ISSR markers were carried out in seven cenopopulations (CP) of R. rosea in 2018 and 2020. The research was founded on the notion of conducting a comparative analysis of the morphogenetic structure of Rhodiola rosea populations in various ecological and geographical conditions of AR. The aim of this work is to evaluate the variability of morphometric traits of sexually mature living female R. rosea plants and to conduct a comparative analysis of genetic variability in cenopopulations (CP) both under undisturbed conditions and under stressful conditions of anthropogenic impact (grazing). Of the 8 primers used, HB12 turned out to be the most informative. The percentage of polymorphic loci in the populations between 0 and 88%. Two populations, located in favorable conditions at relatively low absolute altitudes (2000 m above sea level) (masl) in the undisturbed habitats of the Katun and Altai reserves of AR, were characterized by higher polymorphism. The share of polymorphic loci reached 80%. According to the analysis of statistical data, the highest values of morphometric parameters of the aerial parts of R. rosea plants and the highest potential seed productivity were also recorded in these habitats. Representatives of two high-mountain CPs (2400–2500 masl) in the Sailyugemsky National Park (SNP) were characterized by the lowest genetic polymorphism. Their genetic structure is the most homogeneous, since we have not found polymorphic loci. Due to spatial isolation, these individuals are reliably genetically differentiated. In addition, individuals of one type were subjected to stressful anthropogenic impact (grazing). Therefore, the smallest sizes and lowest potential seed productivity were recorded. Our research shows that alpine populations of R. rosea in AR, under conditions of anthropogenic stress, need protection for their gene pool.
Funder
Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences “Ecosystems of herbaceous pine and small-leaved forests as regulators of nitrogen and carbon balance in the forest-steppe landscape of Western Siberia”
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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