Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
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Published:2023-09-19
Issue:9
Volume:9
Page:944
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
Wang Huiling1,
Yang Kuan1,
Huang Hongping1,
Guo Liwei1,
He Xiahong12
Affiliation:
1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China
2. School of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China
Abstract
Round spot is a destructive disease that limits of Panax notoginseng production in China. However, the genetic diversity of its etiological agent Mycocentrospora acerina has yet to be studied. In this work, firstly, we developed 32 M. acerina polymorphic microsatellite markers using MISA and CERVUS 3.0 and selected 14 for further analysis. Then, we studied the genetic diversity of 187 isolates collected from P. notoginseng round spot using simple sequence repeat markers and polyacrylamide gel electrophoresis. The genetic diversity ranged from 0.813 to 0.946, with 264 alleles detected at the 14 microsatellite loci. The expected average heterozygosity was 0.897.
Funder
China Agriculture Research System of MOF and MARA
Major Science and Technology Project of Yunnan and Kunming
National and Provincial Excellent Scientist Supporting Programme
Innovative Research Team of Science and Technology in Yunnan Province
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
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