Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes

Author:

Sawicki Jakub1ORCID,Krawczyk Katarzyna1ORCID,Paukszto Łukasz1,Maździarz Mateusz1ORCID,Kurzyński Mateusz1,Szablińska-Piernik Joanna1ORCID,Szczecińska Monika1

Affiliation:

1. Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, Poland

Abstract

In this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mitochondrial genomes and around 80 chloroplast genomes. These figures not only demonstrate the technology’s robustness but also mark a substantial advancement in the field, highlighting its efficacy in decoding the complex and dynamic nature of these genomes. Nanopore sequencing, known for its long-read capabilities, significantly surpasses traditional sequencing techniques, especially in addressing challenges like structural complexity and sequence repetitiveness in organellar DNA. This review delves into the nuances of nanopore sequencing, elaborating on its benefits compared to conventional methods and the groundbreaking applications it has fostered in plant organellar genomics. While its transformative impact is clear, the technology’s limitations, including error rates and computational requirements, are discussed, alongside potential solutions and prospects for technological refinement.

Funder

The National Science Center Kraków, Poland

Publisher

MDPI AG

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