Knockdown of klotho Leads to Cell Movement Impairment during Zebrafish Gastrulation

Author:

Pan Heng-Chih1234ORCID,Lo Kang-Chieh5,Wang Yun-Hsin56,Huang Han-Ting5,Cheng Shu-Chun1,Sun Chiao-Yin12,Chen Yau-Hung5ORCID

Affiliation:

1. Division of Nephrology, Department of Internal Medicine, Keelung Chang Gung Memorial Hospital, Keelung 204, Taiwan

2. College of Medicine, Chang Gung University, Taoyuan 333, Taiwan

3. Community Medicine Research Center, Keelung Chang Gung Memorial Hospital, Keelung 204, Taiwan

4. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan

5. Department of Chemistry, Tamkang University, No. 151, Ying-Chuan Road, Tamsui, New Taipei City 251, Taiwan

6. Department of Medical Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei 112, Taiwan

Abstract

(1) Background: Klotho is a multifunctional protein, but its biophysiological roles during fish early development are unclear. (2) Methods: We carried out anti-sense Klotho morpholino injection and whole-mount in situ hybridization experiments in this study. (3) Results: Our results showed that in the Klotho MO1-injected group, embryos displayed longitudinal shapes and swelling yolks compared to those of the un-injected groups. Their length/width ratio by 9 hpf for the uninfected group is 1 ± 0.1; but for 0.1 mM-, 0.2 mM-, and 0.5 mM-MO-injected are 1.1 ± 0.1, 1.2 ± 0.1, and 1.3 ± 0.1, respectively, suggesting that the klotho knockdown-induced length/width ratio changes are in a dose-dependent manner. Whole-mount in situ hybridization showed that several cell migration-related gene expressions, including goosecoid, floating head, bone morphogenetic protein 4, and even-skipped-liked 1 were affected in the Klotho MO-injected embryos. (4) Conclusions: We suggest that knockdown of klotho leads to cell movement impairment during zebrafish gastrulation.

Funder

Chang Gung Memorial Hospital

Publisher

MDPI AG

Subject

Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics

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