Stachys Species: Comparative Evaluation of Phenolic Profile and Antimicrobial and Antioxidant Potential
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Published:2023-11-20
Issue:11
Volume:12
Page:1644
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ISSN:2079-6382
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Container-title:Antibiotics
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language:en
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Short-container-title:Antibiotics
Author:
Benedec Daniela1ORCID, Oniga Ilioara1ORCID, Hanganu Daniela1, Tiperciuc Brîndușa2ORCID, Nistor Adriana1, Vlase Ana-Maria3, Vlase Laurian4ORCID, Pușcaș Cristina5, Duma Mihaela6, Login Cristian Cezar7, Niculae Mihaela8ORCID, Silaghi-Dumitrescu Radu5ORCID
Affiliation:
1. Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania 2. Department of Pharmaceutical Chemistry, “Iuliu Hațieganu” University of Medicine and Pharmacy, 41 V. Babeş Street, 400012 Cluj-Napoca, Romania 3. Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania 4. Department of Pharmaceutical Technology and Biopharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 41 V. Babeş Street, 400012 Cluj-Napoca, Romania 5. Department of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 A. Janos Street, 400028 Cluj-Napoca, Romania 6. State Animal Health and Safety Veterinary Laboratory, 1 Piata Marasti Street, 400609 Cluj-Napoca, Romania 7. Department of Physiology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania 8. Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
Abstract
This study aimed to investigate the polyphenolic composition and antioxidant and antimicrobial potential of six Romanian Stachys species: S. officinalis, S. germanica, S. byzantina, S. sylvatica, S. palustris, and S. recta. The LC-MS/MS method was used to analyze the polyphenolic profile, while the phenolic contents were spectrophotometrically determined. The antioxidant activity was evaluated using the following methods: DPPH, FRAP, nitrite-induced autooxidation of hemoglobin, inhibition of cytochrome c-catalyzed lipid peroxidation, and electron paramagnetic resonance spectroscopy. The in vitro antimicrobial properties were assessed using agar-well diffusion, broth microdilution, and antibiofilm assays. Fifteen polyphenols were identified using LC-MS and chlorogenic acid was the major component in all the samples (1131.8–6761.4 μg/g). S. germanica, S. palustris, and S. byzantina extracts each displayed an intense antiradical action in relation to high contents of TPC (6.40 mg GAE/mL), flavonoids (3.90 mg RE/mL), and caffeic acid derivatives (0.89 mg CAE/mL). In vitro antimicrobial and antibiofilm properties were exhibited towards Candida albicans, Gram-positive and Gram-negative strains, with the most intense efficacy recorded for S. germanica and S. byzantina when tested against S. aureus. These results highlighted Stachys extracts as rich sources of bioactive compounds with promising antioxidant and antimicrobial efficacies and important perspectives for developing phytopharmaceuticals.
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
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