Essential Oils from Mediterranean Plants Inhibit In Vitro Monocyte Adhesion to Endothelial Cells from Umbilical Cords of Females with Gestational Diabetes Mellitus
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Published:2023-04-13
Issue:8
Volume:24
Page:7225
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Schiavone Valeria12, Romasco Tea12ORCID, Di Pietrantonio Nadia12ORCID, Garzoli Stefania3ORCID, Palmerini Carola12, Di Tomo Pamela12ORCID, Pipino Caterina12ORCID, Mandatori Domitilla12, Fioravanti Rossella3, Butturini Elena4, Sabatino Manuela5ORCID, Baldassarre Maria Pompea Antonia26ORCID, Ragno Rino5ORCID, Pandolfi Assunta12ORCID, Di Pietro Natalia12ORCID
Affiliation:
1. Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy 2. Center for Advanced Studies and Technology—CAST, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy 3. Department of Pharmaceutical Chemistry and Technology, Sapienza University of Rome, 00185 Roma, Italy 4. Department of Neurosciences, Biomedicine and Movement Sciences, Section of Biochemistry, University of Verona, 37129 Verona, Italy 5. Rome Center for Molecular Design-RCMD, Department of Pharmaceutical Chemistry and Technology, Sapienza University of Rome, 00185 Roma, Italy 6. Department of Medicine and Aging Sciences, “G. d’Annunzio” University Chieti-Pescara, 66100 Chieti, Italy
Abstract
Essential oils (EOs) are mixtures of volatile compounds belonging to several chemical classes derived from aromatic plants using different distillation techniques. Recent studies suggest that the consumption of Mediterranean plants, such as anise and laurel, contributes to improving the lipid and glycemic profile of patients with diabetes mellitus (DM). Hence, the aim of the present study was to investigate the potential anti-inflammatory effect of anise and laurel EOs (AEO and LEO) on endothelial cells isolated from the umbilical cord vein of females with gestational diabetes mellitus (GDM-HUVEC), which is a suitable in vitro model to reproduce the pro-inflammatory phenotype of a diabetic endothelium. For this purpose, the Gas Chromatographic/Mass Spectrometric (GC-MS) chemical profiles of AEO and LEO were first analyzed. Thus, GDM-HUVEC and related controls (C-HUVEC) were pre-treated for 24 h with AEO and LEO at 0.025% v/v, a concentration chosen among others (cell viability by MTT assay), and then stimulated with TNF-α (1 ng/mL). From the GC-MS analysis, trans-anethole (88.5%) and 1,8-cineole (53.9%) resulted as the major components of AEO and LEO, respectively. The results in C- and GDM-HUVEC showed that the treatment with both EOs significantly reduced: (i) the adhesion of the U937 monocyte to HUVEC; (ii) vascular adhesion molecule-1 (VCAM-1) protein and gene expression; (iii) Nuclear Factor-kappa B (NF-κB) p65 nuclear translocation. Taken together, these data suggest the anti-inflammatory efficacy of AEO and LEO in our in vitro model and lay the groundwork for further preclinical and clinical studies to study their potential use as supplements to mitigate vascular endothelial dysfunction associated with DM.
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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