Evaluation of a Set of miRNAs in 26 Cases of Fatal Traumatic Brain Injuries
-
Published:2023-06-29
Issue:13
Volume:24
Page:10836
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Bonin Serena1ORCID, D’Errico Stefano1, Medeot Caterina1ORCID, Moreschi Carlo2, Ciglieri Solange Sorçaburu1, Peruch Michela1, Concato Monica1, Azzalini Eros1ORCID, Previderè Carlo3, Fattorini Paolo1ORCID
Affiliation:
1. DSM—Department of Medical Sciences, University of Trieste, 34149 Trieste, Italy 2. DAME—Department of Medical Area, University of Udine, 33100 Udine, Italy 3. Department of Public Health, Experimental, and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, 27100 Pavia, Italy
Abstract
In forensic medicine, identifying novel biomarkers for use as diagnostic tools to ascertain causes of death is challenging because of sample degradation. To that aim, a cohort (n = 26) of fatal traumatic brain injuries (TBIs) were tested for three candidate miRNAs (namely, miR-124-3p, miR-138-5p, and miR144-3p). For each case, three FFPE specimens (coup area (CA), contrecoup area (CCA), and the corpus callosum (CC)) were investigated, whereas the FFPE brain tissues of 45 subjects (deceased due to acute cardiovascular events) were used as controls. Relative quantification via the ∆∆Ct method returned significantly higher expression levels of the three candidate miRNAs (p < 0.01) in the TBI cases. No difference was detected in the expression levels of any miRNA investigated in the study among the CA, CCA, and CC. Furthermore, the analyzed miRNAs were unrelated to the TBI samples’ post-mortem intervals (PMIs). On the contrary, has-miR-124-3p ahashsa-miR-144-3p were significantly correlated (p < 0.01) with the agonal time in TBI deaths. Since the RNA was highly degraded in autoptic FFPE tissues, it was impossible to analyze the mRNA targets of the miRNAs investigated in the present study, highlighting the necessity of standardizing pre-analytical processes even for autopsy tissues.
Funder
Regione Friuli Venezia Giulia
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference46 articles.
1. Estimating the global incidence of traumatic brain injury;Dewan;J. Neurosurg.,2018 2. Position statement: Definition of traumatic brain injury;Menon;Arch. Phys. Med. Rehabil.,2010 3. Saukko, P.J., and Knight, B. (2016). Knight’s Forensic Pathology, CRC Press. 4. Aromatario, M., Torsello, A., D’Errico, S., Bertozzi, G., Sessa, F., Cipolloni, L., and Baldari, B. (2021). Traumatic Epidural and Subdural Hematoma: Epidemiology, Outcome, and Dating. Medicina, 57. 5. Livieri, T., Cuttaia, C., Vetrini, R., Concato, M., Peruch, M., Neri, M., Radaelli, D., and D’Errico, S. (2022). Old and Promising Markers Related to Autophagy in Traumatic Brain Injury. Int. J. Mol. Sci., 24.
|
|