Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Author:

Chang Cheng12,He Xiaoyun1ORCID,Di Ran1ORCID,Wang Xiangyu1ORCID,Han Miaoceng2,Liang Chen2ORCID,Chu Mingxing1ORCID

Affiliation:

1. State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China

2. College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Abstract

Litter size is an economically important trait in sheep, and it is a complex trait controlled by multiple genes in multiple organs. Among them, the regulation of lamb number trait by the thyroid gland is a very important part. However, the molecular mechanisms of the thyroid gland in sheep reproduction remain unclear. Here, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) STH sheep, respectively, and to identify differentially expressed circRNAs (DECs) associated with reproduction. Bioinformatic analysis of the source genes of these DECs revealed that they can be enriched in multiple signaling pathways involved in the reproductive process of animals. We found that the source genes of these DECs, such as GNAQ, VEGFC, MAPK1, STAT1, and HSD17B7, may play important roles in the reproductive process of animals. To better understand the function of these DECs, we constructed circRNA–miRNA co-expression networks. Dual luciferase reporter assays suggested that a ceRNA regulatory mechanism between circ_0003259-oar-miR-133-TXLNA and circ_0012128-oar-miR-370-3p-FGFR1 may hold. All of these DEC expression profiles in the thyroid gland provide a novel resource for elucidating the regulatory mechanisms underlying STH sheep prolificacy.

Funder

National Natural Science Foundation of China

China Agriculture Research System of MOF and MARA

Central Public-Interest Scientific Institution Basal Research Fund

Agricultural Science and Technology Innovation Program of China

Open Project of Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design

General Project of Shanxi Province Basic Research Program

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

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