Styrene Production in Genetically Engineered Escherichia coli in a Two-Phase Culture
Author:
Noda Shuhei12ORCID, Fujiwara Ryosuke3ORCID, Mori Yutaro4ORCID, Dainin Mayumi3, Shirai Tomokazu3ORCID, Kondo Akihiko13
Affiliation:
1. Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan 2. PRESTO, Japan Science and Technology Agency (JST), Kawaguchi 332-0012, Saitama, Japan 3. Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 4. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan
Abstract
Styrene is an important industrial chemical. Although several studies have reported microbial styrene production, the amount of styrene produced in batch cultures can be increased. In this study, styrene was produced using genetically engineered Escherichia coli. First, we evaluated five types of phenylalanine ammonia lyases (PALs) from Arabidopsis thaliana (AtPAL) and Brachypodium distachyon (BdPAL) for their ability to produce trans-cinnamic acid (Cin), a styrene precursor. AtPAL2-expressing E. coli produced approximately 700 mg/L of Cin and we found that BdPALs could convert Cin into styrene. To assess styrene production, we constructed an E. coli strain that co-expressed AtPAL2 and ferulic acid decarboxylase from Saccharomyces cerevisiae. After a biphasic culture with oleyl alcohol, styrene production and yield from glucose were 3.1 g/L and 26.7% (mol/mol), respectively, which, to the best of our knowledge, are the highest values obtained in batch cultivation. Thus, this strain can be applied to the large–scale industrial production of styrene.
Funder
RIKEN Center for Sustainable Resource Science JST-Mirai Program Special Postdoctoral Researcher Program Incentive Research Projects JSPS KAKENHI Grant-in-Aid for Scientific Research JST ACT-X
Subject
Applied Microbiology and Biotechnology,Biomedical Engineering,Biochemistry,Bioengineering,Biotechnology
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