Affiliation:
1. Department of Agricultural Life Science, Sunchon National University, Suncheon 59722, Republic of Korea
Abstract
Protopanaxadiol (PPD), a gut microbiome-induced ginseng metabolite, has positive immune effects. We previously reported the immune-boosting and anti-inflammatory effects of PPD-enricshed rice seed extracts in normal and inflammatory cell environments, respectively. In the present study, the immunomodulatory activity of PPD-enriched transgenic rice seed extract (DJ-PPD), which exhibited the highest immune-related activity among all available extracts, was compared with that of commercially synthesized 20s-PPD (S-PPD) and natural ginseng root extract (GE), in RAW264.7 cells. Compared with S-PPD and GE treatment, DJ-PPD treatment (i) significantly promoted NF-κB p65 and c-Jun N-terminal protein kinase (JNK) phosphorylation; (ii) upregulated IL-1β, IL-6, COX-2, TLR-4, and TNF-α expression; (iii) and increased prostaglandin E2 (PGE2) production. However, there were no significant differences in the effects of the three treatments containing PPD-type sapogenin or saponins on nitric oxide (NO) production and phagocytic activity. In the inflammatory cell environment, DJ-PPD treatment markedly decreased the production of LPS-induced inflammatory factors, including NO and PGE2, as well as proinflammatory cytokine expression, by decreasing phosphorylated (p-)NF-κB p65, p-p38 MAPK, and p-JNK levels. Thus, DJ-PPD that does not require complex intestinal microbial processes to exert higher anti-inflammatory effects compared with S-PPD and GE. However, DJ-PPD exerted similar or higher immune-boosting effects (depending on inflammatory biomarkers) than S-PPD and GE. These findings indicate the potential of PPD-enriched transgenic rice as an alternative immunomodulatory agent.
Funder
National Research Foundation of Korea
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference41 articles.
1. Varsha, K.K., Narisetty, V., Brar, K.K., Madhavan, A., Alphy, M.P., Sindhu, R., Awasthi, M.K., Varjani, S., and Binod, P. (2022). Bioactive metabolites in functional and fermented foods and their role as immunity booster and anti-viral innate mechanisms. J. Food Sci. Technol., 1–10.
2. Nutraceuticals of anti-inflammatory activity as complementary therapy for rheumatoid arthritis;Toxicol. Ind. Health,2014
3. Reactive Oxygen Species, Aging, and Antioxidative Nutraceuticals;Lee;Compr. Rev. Food Sci. Food Saf.,2004
4. Nutraceuticals—A regulatory review;Ganesh;Int. J. Drug Regul. Aff.,2018
5. The in Vitro Structure-Related Anti-Cancer Activity of Ginsenosides and Their Derivatives;Dong;Molecules,2011
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