Quantitative Microbial Risk Assessment of North Carolina Type 2 Reclaimed Water for Agricultural Reuse

Abstract

As treated wastewater is increasingly used for agricultural purposes; questions remain about the microbiological quality of produce irrigated by these waters. This study conducted a quantitative microbial risk assessment (QMRA) using microbial data collected from North Carolina Type 2 reclaimed waters, which have been proposed as supplemental irrigation waters. Reclaimed waters were collected from four different water reclamation facilities located in central North Carolina and evaluated for five representative pathogens from the three groups of microorganisms (bacteria, virus, and protozoan parasites). Using these data, produce consumption scenarios were evaluated using a variety of irrigation techniques, including spray irrigation, drip irrigation, and subsurface drip irrigation, and the disability adjusted life years (DALYs) that result from illness by each pathogen as a result of produce consumption were compared to the acceptable level set by the World Health Organization. Based on the types of crop irrigation examined in this study using NC Type 2 reclaimed water, there were irrigation conditions and certain pathogens for which the annual risk of infection was not always reduced below the acceptable DALY risk level of <1 × 10−6 set by the WHO. The risks of viral infection by adenoviruses groups A–F were below the acceptable risk level; however, for Salmonella spp., Cryptosporidium, and Giardia, the annual risk of infection was sometimes greater than would be considered acceptable.