Identification of Intestinal Microbial Community in Gallstone Patients with Metagenomic Next-Generation Sequencing
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Published:2023-08-21
Issue:16
Volume:13
Page:2712
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ISSN:2075-4418
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Container-title:Diagnostics
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language:en
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Short-container-title:Diagnostics
Author:
Ding Li123, Wang Su123ORCID, Jiang Wenrong123, Miao Yingxin123, Liu Wenjian123, Yang Feng123, Zhang Jinghao123, Chi Wenjing123, Liu Tao1, Liu Yue123, Wang Shiwen123, Zhang Yanmei123, Zhao Hu123
Affiliation:
1. Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China 2. Department of Laboratory Medicine, Research Center on Aging and Medicine, Fudan University, Shanghai 200040, China 3. Department of Laboratory Medicine, Huadong Hospital, Shanghai 200040, China
Abstract
Gallstone disease (GD) is one of the most common gastrointestinal diseases worldwide. Nowadays, intestinal microbiota are thought to play important roles in the formation of gallstones. In our study, human fecal samples were extracted for metagenomic next-generation sequencing (mNGS) on the Illumina HiSeq platform, followed by bioinformatics analyses. Our results showed that there was a particular intestinal micro-ecosystem in GD patients. In contrast to healthy people, the sequences of Bacteroidetes, Bacteroides and Thetaiotaomicron were obviously more abundant in GD patients at phylum, genus and species levels, respectively. On the other hand, the glycan metabolism and drug resistance, especially for the β-lactams, were the most profound functions of gut microbes in GD patients compared to those in normal subjects. Furthermore, a correlation analysis drew out that there existed a significant relationship between the serum levels of biochemical indicators and abundances of intestinal microbes in GD patients. Our results illuminate both the composition and functions of intestinal microbiota in GD patients. All in all, our study can broaden the insight into the potential mechanism of how gut microbes affect the progression of gallstones to some extent, which may provide potential targets for the prevention, diagnosis or treatment of GD.
Funder
National Natural Science Foundation of China Scientific Research Project of Shanghai Science and Technology Commission Shanghai Technological Innovation Action Projects Shanghai health and family planning commission youth project of scientific research subject Key Subject of Huadong Hospital Clinical Research Project of Huadong Hospital Shanghai Rising-Star Program Medical Scientific Research Foundation of Guangdong Province
Subject
Clinical Biochemistry
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