Abstract
Two-Spotted Spider Mites (TSSMs, Tetranychus urticae Koch 1836 (Acari: Tetranychidae)) is one of the most important pests in many crop plants, and their feeding activity is based on sucking leaf cell contents. The purpose of this study was to evaluate the interaction between TSSMs and their host Lima bean (Phaseolus lunatus) by analyzing the metabolomics of leaf pigments and the transcriptomics of TSSM guanine production. We also used epifluorescence, confocal laser scanning, and transmission electron microscopies to study the morphology and structure of TSSMs and their excreta. Finally, we evaluated the potential photosynthetic ability of TSSMs and the activity and content of Ribulose-1,5-bisphosphate Carboxylase/Oxigenase (RubisCO). We found that TSSMs express several genes involved in guanine production, including Guanosine Monophosphate Synthetase (GMPS) and decoyinine (DCY), a potential inhibitor of GMPS, was found to reduce TSSMs proliferation in infested Lima bean leaves. Despite the presence of intact chloroplasts and chlorophyll in TSSMs, we demonstrate that TSSMs do not retain any photosynthetic activity. Our results show for the first time the transcriptomics of guanine production in TSSMs and provide new insight into the catabolic activity of TSSMs on leaf chlorophyll and carotenoids. Finally, we preliminary demonstrate that DCY has an acaricidal potential against TSSMs.
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference56 articles.
1. Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: A review;Vontas;Insect Biochem. Mol. Biol.,2010
2. Helle, H., and Sabelis, M.W. (1985). Spider Mites. Their Biology, Natural Enemies and Control, Elsevier.
3. Tetranins: New putative spider mite elicitors of host plant defense;Iida;New Phytol.,2019
4. Compared Biology and behavior of Tetranychus urticae Koch (Acari: Tetranychidae) and Phytoseiulus macropilis (Banks) (Acari: Phytoseiidae) on Bollgard (TM) and non-transgenic isoline cotton;Torres;Neotrop. Entomol.,2010
5. Chlorophyll and its degradation products in the two-spotted spider mite, Tetranychus urticae: Observations using epifluorescence and confocal laser scanning microscopy;Occhipinti;Exp. Appl. Acarol.,2013
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献