ALK, ROS1, RET and NTRK1–3 Gene Fusions in Colorectal and Non-Colorectal Microsatellite-Unstable Cancers
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Published:2023-09-02
Issue:17
Volume:24
Page:13610
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Mulkidjan Rimma S.1, Saitova Evgeniya S.1, Preobrazhenskaya Elena V.12ORCID, Asadulaeva Karimat A.1, Bubnov Mikhail G.1ORCID, Otradnova Ekaterina A.1ORCID, Terina Darya M.1, Shulga Sofia S.1, Martynenko Darya E.1, Semina Maria V.1, Belogubova Evgeniya V.1, Tiurin Vladislav I.1ORCID, Amankwah Priscilla S.1, Martianov Aleksandr S.12ORCID, Imyanitov Evgeny N.12ORCID
Affiliation:
1. Department of Tumor Growth Biology, N.N. Petrov Institute of Oncology, 197758 St. Petersburg, Russia 2. Department of Medical Genetics, St. Petersburg Pediatric Medical University, 194100 St. Petersburg, Russia
Abstract
This study aimed to conduct a comprehensive analysis of actionable gene rearrangements in tumors with microsatellite instability (MSI). The detection of translocations involved tests for 5′/3′-end expression imbalance, variant-specific PCR and RNA-based next generation sequencing (NGS). Gene fusions were detected in 58/471 (12.3%) colorectal carcinomas (CRCs), 4/69 (5.8%) gastric cancers (GCs) and 3/65 (4.6%) endometrial cancers (ECs) (ALK: 8; RET: 12; NTRK1: 24; NTRK2: 2; NTRK3: 19), while none of these alterations were observed in five cervical carcinomas (CCs), four pancreatic cancers (PanCs), three cholangiocarcinomas (ChCs) and two ovarian cancers (OCs). The highest frequency of gene rearrangements was seen in KRAS/NRAS/BRAF wild-type colorectal carcinomas (53/204 (26%)). Surprisingly, as many as 5/267 (1.9%) KRAS/NRAS/BRAF-mutated CRCs also carried tyrosine kinase fusions. Droplet digital PCR (ddPCR) analysis of the fraction of KRAS/NRAS/BRAF mutated gene copies in kinase-rearranged tumors indicated that there was simultaneous co-occurrence of two activating events in cancer cells, but not genetic mosaicism. CRC patients aged above 50 years had a strikingly higher frequency of translocations as compared to younger subjects (56/365 (15.3%) vs. 2/106 (1.9%), p = 0.002), and this difference was particularly pronounced for tumors with normal KRAS/NRAS/BRAF status (52/150 (34.7%) vs. 1/54 (1.9%), p = 0.001). There were no instances of MSI in 56 non-colorectal tumors carrying ALK, ROS1, RET or NTRK1 rearrangements. An analysis of tyrosine kinase gene translocations is particularly feasible in KRAS/NRAS/BRAF wild-type microsatellite-unstable CRCs, although other categories of tumors with MSI also demonstrate moderate occurrence of these events.
Funder
Russian Science Foundation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference31 articles.
1. The diversity of tumours with microsatellite instability: Molecular mechanisms and impact upon microsatellite instability testing and mismatch repair protein immunohistochemistry;Shia;Histopathology,2020 2. Comprehensive analysis of oncogenic fusions in mismatch repair deficient colorectal carcinomas by sequential DNA and RNA next generation sequencing;Wang;J. Transl. Med.,2021 3. Gallon, R., Gawthorpe, P., Phelps, R.L., Hayes, C., Borthwick, G.M., Santibanez-Koref, M., Jackson, M.S., and Burn, J. (2021). How Should We Test for Lynch Syndrome? A Review of Current Guidelines and Future Strategies. Cancers, 13. 4. Deficient mismatch repair/microsatellite unstable colorectal cancer: Diagnosis, prognosis and treatment;Taieb;Eur. J. Cancer,2022 5. Microsatellite instability and immune checkpoint inhibitors: Toward precision medicine against gastrointestinal and hepatobiliary cancers;Eso;J. Gastroenterol.,2019
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