Transcriptome Analysis Reveals POD as an Important Indicator for Assessing Low-Temperature Tolerance in Maize Radicles during Germination

Author:

Zhang Yifei123,Li Jiayu1,Li Weiqing1,Gao Xinhan1,Xu Xiangru1,Zhang Chunyu123,Yu Song123ORCID,Dou Yi1,Luo Wenqi1,Yu Lihe123

Affiliation:

1. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China

2. Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement, Daqing 163319, China

3. Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs, Daqing 163319, China

Abstract

Low-temperature stress (TS) limits maize (Zea mays L.) seed germination and agricultural production. Exposure to TS during germination inhibits radicle growth, triggering seedling emergence disorders. Here, we aimed to analyse the changes in gene expression in the radicles of maize seeds under TS by comparing Demeiya1 (DMY1) and Zhengdan958 (ZD958) (the main Northeast China cultivars) and exposing them to two temperatures: 15 °C (control) and 5 °C (TS). TS markedly decreased radicle growth as well as fresh and dry weights while increasing proline and malondialdehyde contents in both test varieties. Under TS treatment, the expression levels of 5301 and 4894 genes were significantly different in the radicles of DMY1 and ZD958, respectively, and 3005 differentially expressed genes coexisted in the radicles of both varieties. The phenylpropanoid biosynthesis pathway was implicated within the response to TS in maize radicles, and peroxidase may be an important indicator for assessing low-temperature tolerance during maize germination. Peroxidase-encoding genes could be important candidate genes for promoting low-temperature resistance in maize germinating radicles. We believe that this study enhances the knowledge of mechanisms of response and adaptation of the maize seed germination process to TS and provides a theoretical basis for efficiently assessing maize seed low-temperature tolerance and improving maize adversity germination performance.

Funder

National Key Research and Development Program of China

Postdoctoral Science Foundation Funded General Project of Heilongjiang Province

University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province

Heilongjiang Bayi Agricultural Reclamation University Graduate Innovation Research Project

Publisher

MDPI AG

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