Histone Acetyltransferase Rtt109 Regulates Development, Morphogenesis, and Citrinin Biosynthesis in Monascus purpureus
-
Published:2023-04-29
Issue:5
Volume:9
Page:530
-
ISSN:2309-608X
-
Container-title:Journal of Fungi
-
language:en
-
Short-container-title:JoF
Author:
Shi Ruoyu12,
Gong Pengfei1,
Luo Qiaoqiao1,
Chen Wei1ORCID,
Wang Chengtao1
Affiliation:
1. Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China
2. Yunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming 650201, China
Abstract
Histone acetyltransferase (HAT) has been reported to be pivotal for various physiological processes in many fungi. However, the functions that HAT Rtt109 perform in edible fungi Monascus and the underlying mechanism remains unclear. Here, we identified the rtt109 gene in Monascus, constructed the rtt109 knockout strain (Δrtt109) and its complementary strain (Δrtt109:com) by CRISPR/Cas9 methods, and functionally characterized the roles that Rtt109 play in Monascus. Deletion of rtt109 significantly reduced conidia formation and colony growth, whereas, it increased the yield of Monascus pigments (MPs) and citrinin (CTN). Further real-time quantitative PCR (RT-qPCR) analysis indicated that Rtt109 remarkably affected the transcriptional expression of key genes related to development, morphogenesis, and secondary metabolism of Monascus. Together, our results revealed the critical roles of HAT Rtt109 in Monascus, and enriched our current knowledge of the development and regulation of secondary metabolism in fungi, throwing light on restraining or eliminating citrinin in the development and industrial applications of Monascus.
Funder
Beijing Municipal Natural Science Foundation Beijing Municipal Education Commission Science & Technology Plan Key Joint Project
Beijing Engineering Technology Research Center Platform Construction Project
The Construction of High-precision Disciplines in Beijing-Food Science & Engineering
Cultivation Project of Double First-Class Disciplines of Food Science and Engineering, Beijing Technology & Business University
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献