Preparation and Characterization of Boza Enriched with Nonfat Dry Milk and Its Impact on the Fermentation Process

Author:

Pulatsu Ezgi1,Malik Sargun2ORCID,Lin Mengshi3,Krishnaswamy Kiruba34,Vardhanabhuti Bongkosh3

Affiliation:

1. School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, Canada

2. RNAissance AG LLC, Biotechnology Research Overland Park, St. Louis, MO 63132, USA

3. Department of Food, Nutrition & Exercise Sciences, University of Missouri, Columbia, MO 65201, USA

4. Department of Biomedical, Biological and Chemical Engineering, University of Missouri, Columbia, MO 65211, USA

Abstract

Boza is an indigenous, traditional, low-alcohol and highly viscous beverage prepared by fermenting cereals. Its thick and gel-like consistency make it suitable for consumption via spoon. Although boza is a nutritious beverage, its protein content is very low (<2%). A new type of boza was developed by incorporating nonfat dry milk (NFDM) to elevate the protein content of the beverage. Different NFDM amounts (10 to 40% w/v) were added to determine the best concentration and fermentation time based on the refractive index and pH values at room temperature (0–48 h). The best sample was further characterized by rheological analyses and Fourier transform infrared (FTIR) spectroscopy. The sample with 10% NFDM was the best, as fermentation was successfully performed, and further addition of NFDM increased the initial pH. The refractive index and pH decreased from 21.9 ± 0.1 to 11.8 ± 0.1 and 5.77± 0.50 to 4.09 ± 0.35 during fermentation, respectively. The samples exhibited shear-thinning, solid-like behavior, and a gel-like structure. FTIR analysis by independent modeling of class analogy (SIMCA) demonstrated that unfermented slurry and the fermented product could be effectively differentiated. With the addition of 10% NFDM, the increase in the protein content of the boza medium became significant.

Funder

Robert T. Marshall Scholarship for the first author

USDA NIFA

Publisher

MDPI AG

Subject

Polymers and Plastics,Organic Chemistry,Biomaterials,Bioengineering

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