Affiliation:
1. College of Life Sciences, Qingdao University, Qingdao 266071, China
Abstract
The obligate biotrophic fungal pathogen Blumeria graminis forma specialis tritici (B.g. tritici) is the causal agent of wheat powdery mildew disease. The TOPLESS-related 1 (TPR1) corepressor regulates plant immunity, but its role in regulating wheat resistance against powdery mildew remains to be disclosed. Herein, TaTPR1 was identified as a positive regulator of wheat post-penetration resistance against powdery mildew disease. The transient overexpression of TaTPR1.1 or TaTPR1.2 confers wheat post-penetration resistance powdery mildew, while the silencing of TaTPR1.1 and TaTPR1.2 results in an enhanced wheat susceptibility to B.g. tritici. Furthermore, Defense no Death 1 (TaDND1) and Defense no Death 2 (TaDND2) were identified as wheat susceptibility (S) genes facilitating a B.g. tritici infection. The overexpression of TaDND1 and TaDND2 leads to an enhanced wheat susceptibility to B.g. tritici, while the silencing of wheat TaDND1 and TaDND2 leads to a compromised susceptibility to powdery mildew. In addition, we demonstrated that the expression of TaDND1 and TaDND2 is negatively regulated by the wheat transcriptional corepressor TaTPR1. Collectively, these results implicate that TaTPR1 positively regulates wheat post-penetration resistance against powdery mildew probably via suppressing the S genes TaDND1 and TaDND2.
Funder
Natural Science Foundation of Shandong Province
Qingdao Science and Technology Bureau Fund
Qingdao University Fund
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference56 articles.
1. Evolution and origin of bread wheat;Levy;Plant Cell,2022
2. The outlook for population growth;Lee;Science,2011
3. The global burden of pathogens and pests on major food crops;Savary;Nat. Ecol. Evol.,2019
4. Kusch, S., Qian, J., Loos, A., Kümmel, F., Spanu, P.D., and Panstruga, R. (2023). Long-term and rapid evolution in powdery mildew fungi. Mol. Ecol.
5. Combating powdery mildew: Advances in molecular interactions between Blumeria graminis f. sp. tritici and wheat;Mapuranga;Front. Plant Sci.,2022