Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection

Author:

Garnier Nathalie12ORCID,Sane Famara1,Massara Layal3,Soncin Fabrice45ORCID,Gosset Philippe3ORCID,Hober Didier1,Szunerits Sabine2ORCID,Engelmann Ilka16ORCID

Affiliation:

1. Laboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, France

2. Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, F-59000 Lille, France

3. CNRS UMR 9017, Inserm U1019, CHU Lille, Institut Pasteur de Lille, CIIL—OpInfIELD, University Lille, F-59000 Lille, France

4. CNRS/IIS/Centre Oscar Lambret/Lille University SMMiL-E Project, CNRS Délégation Hauts-de-France, F-59000 Lille, France

5. Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, CNRS IRL2820, Tokyo 113-0033, Japan

6. PCCEI, University Montpellier, INSERM, EFS, CHU Montpellier, F-34000 Montpellier, France

Abstract

miRNAs, small non-coding RNAs that regulate gene expression, are involved in various pathological processes, including viral infections. Virus infections may interfere with the miRNA pathway through the inhibition of genes involved in miRNA biogenesis. A reduction in the number and the levels of miRNAs expressed in nasopharyngeal swabs of patients with severe COVID-19 was lately observed by us, pointing towards the potential of miRNAs as possible diagnostic or prognostic biomarkers for predicting outcomes among patients with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The objective of the present study was to investigate whether SARS-CoV-2 infection influences the expression levels of messenger RNAs (mRNAs) of key genes involved in miRNA biogenesis. mRNA levels of AGO2, DICER1, DGCR8, DROSHA, and Exportin-5 (XPO5) were measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) in nasopharyngeal swab specimens from patients with COVID-19 and controls, as well as in cells infected with SARS-CoV-2 in vitro. Our data showed that the mRNA expression levels of AGO2, DICER1, DGCR8, DROSHA, and XPO5 were not significantly different in patients with severe COVID-19 when compared to patients with non-severe COVID-19 and controls. Similarly, the mRNA expression of these genes was not affected by SARS-CoV-2 infection in NHBE and Calu-3 cells. However, in Vero E6 cells, AGO2, DICER1, DGCR8, and XPO5 mRNA levels were slightly upregulated 24 h after infection with SARS-CoV-2. In conclusion, we did not find evidence for downregulation of mRNA levels of miRNA biogenesis genes during SARS-CoV-2 infection, neither ex vivo nor in vitro.

Funder

I-Site ULNE

Centre Hospitalier Universitaire de Lille

Université de Lille

Ecole Doctorale Biologie-Santé

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

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