Extracellular Self- and Non-Self DNA Involved in Damage Recognition in the Mistletoe Parasitism of Mesquite Trees
-
Published:2023-12-29
Issue:1
Volume:25
Page:457
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
López-García Claudia Marina1, Ávila-Hernández César Alejandro2, Quintana-Rodríguez Elizabeth1, Aguilar-Hernández Víctor3ORCID, Lozoya-Pérez Nancy Edith1, Rojas-Raya Mariana Atzhiry2, Molina-Torres Jorge2ORCID, Araujo-León Jesús Alfredo3ORCID, Brito-Argáez Ligia3, González-Sánchez Avel Adolfo4ORCID, Ramírez-Chávez Enrique2, Orona-Tamayo Domancar1ORCID
Affiliation:
1. Medio Ambiente y Biotecnología, Centro de Innovación Aplicada en Tecnologías Competitivas (CIATEC), León 37545, Guanajuato, Mexico 2. Centro de Investigación y de Estudios Avanzados (CINVESTAV), Instituto Politécnico Nacional, Irapuato 36821, Guanajuato, Mexico 3. Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán (CICY), Mérida 97205, Yucatán, Mexico 4. Facultad de Ingeniería, Universidad Autónoma de Yucatán (UADY), Mérida 97203, Yucatán, Mexico
Abstract
Psittacanthus calyculatus parasitizes mesquite trees through a specialized structure called a haustorium, which, in the intrusive process, can cause cellular damage in the host tree and release DAMPs, such as ATP, sugars, RNA, and DNA. These are highly conserved molecules that primarily function as signals that trigger and activate the defense responses. In the present study, we generate extracellular DNA (exDNA) from mesquite (P. laevigata) tree leaves (self-exDNA) and P. calyculatus (non-self exDNA) mistletoe as DAMP sources to examine mesquite trees’ capacity to identify specific self or non-self exDNA. We determined that mesquite trees perceive self- and non-self exDNA with the synthesis of O2•−, H2O2, flavonoids, ROS-enzymes system, MAPKs activation, spatial concentrations of JA, SA, ABA, and CKs, and auxins. Our data indicate that self and non-self exDNA application differs in oxidative burst, JA signaling, MAPK gene expression, and scavenger systems. This is the first study to examine the molecular biochemistry effects in a host tree using exDNA sources derived from a mistletoe.
Funder
CIENCIA PRODUCTIVA 2023-IDEAGTO Ciencia Basica grant Ciencia de Frontera Consejo Nacional de Ciencia y Tecnología
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference126 articles.
1. Análisis técnico del árbol del mezquite (Prosopis laevigata Humb. & Bonpl. ex Willd.) en México;Sauceda;Ra Ximhai,2014 2. Mesquite gum (Prosopis gum): Structure, properties & applications—A review;Mudgil;Int. J. Biol. Macromol.,2020 3. Villalón-Mendoza, H., Hernández-Hernández, E.E., and Manzanares-Miranda, N. (2023). Sustainable Management of Natural Resources: Diversity, Ecology, Taxonomy and Sociology, Springer. 4. Rasanen, L.A., and Lindstrom, K. (2003). Effects of Biotic and Abiotic Constraints on the Symbiosis between Rhizobia and the Tropical Leguminous Trees Acacia and Prosopis, NISCAIR-CSIR. 5. Mesquite (Prosopis juliflora (Sw.) DC.), huisache (Acacia farnesiana (L.) Willd.) and catclaw (Mimosa biuncifera Benth.) and their effect on dynamics of carbon and nitrogen in soils of the semi-arid highlands of Durango Mexico;Herrera;J. Arid. Environ.,2007
|
|