Affiliation:
1. FGBI Federal Centre for Animal Health, 600901 Vladimir, Russia
Abstract
Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination.
Funder
Ministry of Education and Science of Russia
Subject
Biochemistry, Genetics and Molecular Biology (miscellaneous),Structural Biology,Biotechnology
Reference33 articles.
1. Sánchez-Vizcaíno, J.M., Laddomada, A., and Arias, M.L. (2019). Diseases of Swine, Wiley.
2. The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome;Krug;J. Virol.,2015
3. Whole-genome sequencing of African swine fever virus from wild boars in the Kaliningrad region reveals unique and distinguishing genomic mutations;Mazloum;Front. Vet. Sci.,2023
4. Laboratory methods to study African swine fever virus;Bustos;Virus Res.,2013
5. The use of leukocyte cultures for diagnosing African swine fever (ASF);Hess;Bull. Office Intern. Epizoot.,1960