Specific Proteomic Identification of Collagen-Binding Proteins in Escherichia coli O157:H7: Characterisation of OmpA as a Potent Vaccine Antigen

Author:

Monteiro Ricardo123ORCID,Chafsey Ingrid1,Caccia Nelly1,Ageorges Valentin1,Leroy Sabine1,Viala Didier4,Hébraud Michel14ORCID,Livrelli Valérie5,Pizza Mariagrazia26ORCID,Pezzicoli Alfredo2,Desvaux Mickaël1ORCID

Affiliation:

1. INRAE, UCA, UMR0454 MEDIS, 63000 Clermont-Ferrand, France

2. GSK, 53100 Siena, Italy

3. Instituto de Investigação e Inovação em Saúde–i3S, Universidade do Porto, 4150-564 Porto, Portugal

4. INRAE, Metabolism Exploration Platform, Proteomic Component (PFEMcp), 63122 Saint-Genès Champanelle, France

5. UCA, INSERM, INRAE, M2ISH, 63000 Clermont-Ferrand, France

6. Imperial College London, South Kensington Campus, London SW7 2AZ, UK

Abstract

Escherichia coli is a versatile commensal species of the animal gut that can also be a pathogen able to cause intestinal and extraintestinal infections. The plasticity of its genome has led to the evolution of pathogenic strains, which represent a threat to global health. Additionally, E. coli strains are major drivers of antibiotic resistance, highlighting the urgent need for new treatment and prevention measures. The antigenic and structural heterogeneity of enterohaemorrhagic E. coli colonisation factors has limited their use for the development of effective and cross-protective vaccines. However, the emergence of new strains that express virulence factors deriving from different E. coli diarrhoeagenic pathotypes suggests that a vaccine targeting conserved proteins could be a more effective approach. In this study, we conducted proteomics analysis and functional protein characterisation to identify a group of proteins potentially involved in the adhesion of E. coli O157:H7 to the extracellular matrix and intestinal epithelial cells. Among them, OmpA has been identified as a highly conserved and immunogenic antigen, playing a significant role in the adhesion phenotype of E. coli O157:H7 and in bacterial aggregation. Furthermore, antibodies raised against recombinant OmpA effectively reduced the adhesion of E. coli O157:H7 to intestinal epithelial cells. The present work highlights the role of OmpA as a potent antigen for the development of a vaccine against intestinal pathogenic E. coli.

Publisher

MDPI AG

Subject

General Medicine

Reference62 articles.

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2. WHO (2014). Antimicrobial Resistance: Global Report on Surveillance, World Health Organization. Available online: https://www.who.int/drugresistance/documents/surveillancereport/en/.

3. Preventing urinary tract infection: Progress toward an effective Escherichia coli vaccine;Brumbaugh;Expert Rev. Vaccines,2012

4. Vaccines for preventing enterotoxigenic Escherichia coli (ETEC) diarrhoea;Ahmed;Cochrane Database Syst. Rev.,2013

5. Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome;Tarr;Lancet,2005

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