Affiliation:
1. Institut für Lasertechnologien in der Medizin und Meßtechnik, Universität Ulm, Helmholtzstr 12, 89081 Ulm, Germany
Abstract
We present a goniometer designed for capturing spectral and angular-resolved data from scattering and absorbing media. The experimental apparatus is complemented by a comprehensive Monte Carlo simulation, meticulously replicating the radiative transport processes within the instrument’s optical components and simulating scattering and absorption across arbitrary volumes. Consequently, we were able to construct a precise digital replica, or “twin”, of the experimental setup. This digital counterpart enabled us to tackle the inverse problem of deducing optical parameters such as absorption and scattering coefficients, along with the scattering anisotropy factor from measurements. We achieved this by fitting Monte Carlo simulations to our goniometric measurements using a Levenberg–Marquardt algorithm. Validation of our approach was performed using polystyrene particles, characterized by Mie scattering, supplemented by a theoretical analysis of algorithmic convergence. Ultimately, we demonstrate strong agreement between optical parameters derived using our novel methodology and those obtained via established measurement protocols.
Funder
Invest BW (VDI/VDE Innovation + Technik GmbH) within the project Gon2Twin
BMBF (Bundesministerium für Bildung und Forschung) within the project Requant
Reference41 articles.
1. A review of the optical properties of biological tissues;Cheong;IEEE J. Quantum Electron.,1990
2. Review of biomedical optical imaging—A powerful, non-invasive, non-ionizing technology for improving in vivo diagnosis;Balas;Meas. Sci. Technol.,2009
3. Optical properties of biological tissues: A review;Jacques;Phys. Med. Biol.,2013
4. Tromberg, B.J., Anderson, R.R., Birngruber, R., Brinkmann, R., Berns, M.W., Parrish, J.A., and Apiou-Sbirlea, G. (2016). Biomedical optics centers: Forty years of multidisciplinary clinical translation for improving human health. J. Biomed. Opt., 21.
5. Light-scattering methods for tissue diagnosis;Steelman;Optica,2019