Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil
Author:
Cunha Lia1, Luchs Adriana1ORCID, Azevedo Lais S.1, Silva Vanessa C. M.1, Lemos Marcilio F.1, Costa Antonio C.2ORCID, Compri Adriana P.1, França Yasmin1, Viana Ellen1, Malta Fernanda3, Medeiros Roberta S.1, Guiducci Raquel1, Morillo Simone G.1, Gomes-Gouvea Michele S.2, Amgarten Deyvid3, Pinho João R. R.23ORCID, Moreira Regina C.1ORCID
Affiliation:
1. Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil 2. Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil 3. Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil
Abstract
Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in São Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7–93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide.
Funder
Sao Paulo Research Foundation Fundo Especial de Saúde para Imunização em Massa e Controle de Doenças Hospital das Clínicas, Faculty of Medicine, University of São Paulo
Subject
Virology,Infectious Diseases
Reference76 articles.
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