Microheterogeneity and Individual Differences of Human Urinary N-Glycome under Normal Physiological Conditions
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Published:2023-04-27
Issue:5
Volume:13
Page:756
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ISSN:2218-273X
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Container-title:Biomolecules
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language:en
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Short-container-title:Biomolecules
Author:
Zedan Hend1, Morimura Kousuke1, Elguoshy Amr2, Yamamoto Tadashi2, Natsuka Shunji1
Affiliation:
1. Department of Life and Food Sciences, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan 2. Biofluid Biomarker Center, Niigata University, Niigata 950-2181, Japan
Abstract
Urine is considered an outstanding biological fluid for biomarker discovery, reflecting both systemic and urogenital physiology. However, analyzing the N-glycome in urine in detail has been challenging due to the low abundance of glycans attached to glycoproteins compared to free oligosaccharides. Therefore, this study aims to thoroughly analyze urinary N-glycome using LC-MS/MS. The N-glycans were released using hydrazine and labeled with 2-aminopyridine (PA), followed by anion-exchange fractionation before LC-MS/MS analysis. A total of 109 N-glycans were identified and quantified, of which 58 were identified and quantified repeatedly in at least 80% of samples and accounted for approximately 85% of the total urinary glycome signal. Interestingly, a comparison between urine and serum N-glycome revealed that approximately 50% of the urinary glycome could originate from the kidney and urinary tract, where they were exclusively identified in urine, while the remaining 50% were common in both. Additionally, a correlation was found between age/sex and the relative abundances of urinary N-glycome, with more age-related changes observed in women than men. The results of this study provide a reference for human urine N-glycome profiling and structural annotations.
Subject
Molecular Biology,Biochemistry
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