Ingestion of Bean Leaves Reduces Metabolic Complications and Restores Intestinal Integrity in C57BL/6 Mice with Obesity Induced by a High-Fat and High-Fructose Diet
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Published:2024-01-26
Issue:3
Volume:16
Page:367
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Ocampo-Anguiano Perla Viridiana12ORCID, Victoria-Ruiz Laura Lizeth1, Reynoso-Camacho Rosalía1, Olvera-Ramírez Andrea Margarita3ORCID, Rocha-Guzmán Nuria Elizabeth4, Ramos-Gómez Minerva1ORCID, Ahumada-Solórzano Santiaga Marisela2ORCID
Affiliation:
1. Research and Postgraduate Department in Food Science, School of Chemistry, Autonomous University of Queretaro, Centro Universitario, Cerro de las Campanas S/N, Queretaro 76010, Mexico 2. Interdisciplinary Research in Biomedicine, Faculty of Natural Sciences, Autonomous University of Queretaro, Campus Juriquilla, Av. de las Ciencias S/N, Queretaro 76230, Mexico 3. Department of Veterinary Medicine, Faculty of Natural Sciences, Autonomous University of Queretaro, Campus Juriquilla, Av. de las Ciencias S/N, Queretaro 76230, Mexico 4. Research Group on Functional Foods and Nutraceuticals, Department of Chemical and Biochemical Engineering, TecNM/Instituto Tecnológico de Durango, Durango 34080, Mexico
Abstract
Consumption of foods with fiber and compounds can promote gastrointestinal health and reduce obesity complications. Therefore, treatment with common bean leaves (BL) against obesity was evaluated in mice with a high-fat and high-fructose diet (HFFD) for 14 weeks. The bromatological and phytochemical characterization of BL were determined. Afterwards, the animals were supplemented with BL (10%) or a standard diet (SD) as a strategy to encourage a healthy diet for 12 additional weeks. Changes in body composition, lipid profile, and intestinal integrity were analyzed. The characterization of BL stood out for its content of 27.2% dietary fiber, total phenolics (475.04 mg/100 g), and saponins (2.2 mg/100 g). The visceral adipose tissue (VAT) decreased in the BL group by 52% compared to the HFFD group. Additionally, triglyceride levels were 23% lower in the BL consumption group compared to the HFFD group. The improvement in lipid profile was attributed to the 1.77-fold higher fecal lipid excretion in the BL consumption group compared to the HFFD group and the inhibition of pancreatic lipase by 29%. Furthermore, BL supplementation reduced the serum levels of IL-6 (4.4-fold) and FITC–dextran by 50% compared with those in the HFFD group. Metabolic endotoxemia was inhibited after BL supplementation (−33%) compared to the HFFD group. BL consumption as a treatment in obese mice reduces adipose tissue accumulation and improves the lipid profile. Furthermore, we report for the first time that BL consumption improves intestinal integrity.
Funder
the Fondo de Proyectos Especiales de Rectoría-UAQ Fondo para el desarrollo del conocimiento FONDEC 2021 the Consejo Nacional de Humanidades, Ciencia y Tecnología
Subject
Food Science,Nutrition and Dietetics
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