The Impact of Acute Low-Dose Gamma Irradiation on Biomass Accumulation and Secondary Metabolites Production in Cotinus coggygria Scop. and Fragaria × ananassa Duch. Red Callus Cultures
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Published:2023-07-28
Issue:8
Volume:13
Page:894
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ISSN:2218-1989
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Container-title:Metabolites
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language:en
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Short-container-title:Metabolites
Author:
Ciocan Alexandra-Gabriela12, Maximilian Carmen1, Mitoi Elena Monica1ORCID, Moldovan Radu-Cristian3, Neguț Daniel4ORCID, Iuga Cristina-Adela35ORCID, Helepciuc Florența Elena1, Holobiuc Irina1, Radu Mihai6ORCID, Vassu Dimov Tatiana2, Cogălniceanu Gina1
Affiliation:
1. Department of Developmental Biology, Institute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei Street, 060031 Bucharest, Romania 2. Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095 Bucharest, Romania 3. Department of Proteomics and Metabolomics, Research Center for Advanced Medicine—MedFuture, “Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca, 4-6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania 4. IRASM Radiation Processing Department, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului Street 30, 077125 Magurele, Romania 5. Department of Pharmaceutical Analysis, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania 6. Department of Life and Environmental Physics, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului Street 30, 077125 Magurele, Romania
Abstract
Cotinus coggygria Scop. (smoketree) and Fragaria × ananassa Duch. (strawberry) are two industrially important species due to their composition in bioactive compounds. In this study, we investigated the effects of acute low-dose gamma irradiation (15, 20, 25, 30, 35 and 40 Gy) on two red callus cultures established in smoketree and strawberry. The biomass production, dry weight, content of phenols, flavonoids, monomeric anthocyanins’, index of anthocyanins polymerization and antioxidant activity were evaluated. For the smoketree callus, a negative correlation between irradiation doses and callus biomass accumulation was observed. For the strawberry callus, irradiation did not significantly affect the accumulation of the biomass. An increased dry weight was observed in irradiated smoketree callus, while for treated strawberry callus, a decrease was recorded. Irradiation with 30 Gy was stimulative for polyphenols’ accumulation in both cultures; however, the increase was significant only in the strawberry callus. The flavonoids increased in the 30 Gy strawberry variants, while it significantly decreased in smoketree callus irradiated with 35 and 40 Gy. In irradiated strawberry callus, except for the 25 Gy variant (1.65 ± 0.4 mg C-3-GE/g DW), all treatments caused an increase in anthocyanins’ accumulation. In smoketree, except for the 15 Gy variant (2.14 ± 0.66 mg C-3-GE/g DW), the irradiation determined an increase in anthocyanins synthesis, with the highest value being seen in the 20 Gy variant (2.8 ± 0.94 mg C-3-GE/g DW). According to UPLC-HRMS investigations, an unidentified compound increased by 99% at the 30 Gy dose in strawberry callus, while in smoketree, maslinic acid increased by 51% after irradiation with 40 Gy. The results of this study showed, for the first time, the differential response of two performant callus cultures to low-dose gamma irradiation, a biotechnological method that can be used to stimulate the synthesis of important flavonoids and triterpenes.
Funder
Romanian Ministry of Research and Innovation Romanian Academy for Institute of Biology Bucharest of Romanian Academy Doctoral School of Biology
Subject
Molecular Biology,Biochemistry,Endocrinology, Diabetes and Metabolism
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