Affiliation:
1. Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha 61441, Saudi Arabia
2. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al Kharj 11942, Saudi Arabia
Abstract
Niosomes are multilamellar vesicles that effectively transfer active ingredients into the skin’s layers. To improve the active substance’s penetration across the skin, these carriers are frequently utilized as topical drug delivery systems. Essential oils (EOs) have garnered significant interest in the field of research and development owing to their various pharmacological activities, cost-effectiveness, and simple manufacturing techniques. However, these ingredients undergo degradation and oxidation over time, leading to a loss of functionality. Niosome formulations have been developed to deal with these challenges. The main goal of this work was to create a niosomal gel of carvacrol oil (CVC) to improve its penetration into the skin for anti-inflammatory actions and stability. By changing the ratio of drug, cholesterol and surfactant, various formulations of CVC niosomes were formulated using Box Behnken Design (BBD). A thin-film hydration technique using a rotary evaporator was employed for the development of niosomes. Following optimization, the CVC-loaded niosomes had shown: 180.23 nm, 0.265, −31.70 mV, and 90.61% of vesicle size, PDI, zeta potential, and EE%. An in vitro study on drug release discovered the rates of drug release for CVC-Ns and CVC suspension, which were found to be 70.24 ± 1.21 and 32.87 ± 1.03, respectively. The release of CVC from niosomes best fit the Higuchi model, and the Korsmeyer–Peppas model suggests that the release of the drug followed the non-Fickian diffusion. In a dermatokinetic investigation, niosome gel significantly increased CVC transport in the skin layers when compared to CVC–conventional formulation gel (CVC-CFG). Confocal laser scanning microscopy (CLSM) of rat skin exposed to the rhodamine B-loaded niosome formulation showed a deeper penetration of 25.0 µm compared to the hydroalcoholic rhodamine B solution (5.0 µm). Additionally, the CVC-N gel antioxidant activity was higher than that of free CVC. The formulation coded F4 was selected as the optimized formulation and then gelled with carbopol to improve its topical application. Niosomal gel underwent tests for pH determination, spreadability, texture analysis, and CLSM. Our findings imply that the niosomal gel formulations could represent a potential strategy for the topical delivery of CVC in the treatment of inflammatory disease.
Funder
Deanship of Scientific Research at King Khalid University
Subject
Polymers and Plastics,Organic Chemistry,Biomaterials,Bioengineering
Reference71 articles.
1. De Microencapsulation: Its Application in Nutrition;Schrooyen;Proc. Nutr. Soc.,2001
2. Souto, E.B., Fernandes, A.R., Martins-Gomes, C., Coutinho, T.E., Durazzo, A., Lucarini, M., Souto, S.B., Silva, A.M., and Santini, A. (2020). Nanomaterials for Skin Delivery of Cosmeceuticals and Pharmaceuticals. Appl. Sci., 10.
3. Art of prevention: Essential oils-natural products not necessarily safe;Sindle;Int. J. Women’s Dermatol.,2021
4. Effect of Preparation Methods on the Properties of Zataria Multiflora Boiss. Essential Oil Loaded Nanoliposomes: Characterization of Size, Encapsulation Efficiency and Stability;Khatibi;Pharm. Sci.,2015
5. Two-Step Method for Encapsulation of Oregano Essential Oil in Chitosan Nanoparticles: Preparation, Characterization and in Vitro Release Study;Hosseini;Carbohydr. Polym.,2013
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