An Innovative Fluid Dynamic System to Model Inflammation in Human Skin Explants
-
Published:2023-03-27
Issue:7
Volume:24
Page:6284
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Galvan Andrea1ORCID, Cappellozza Enrica1, Pellequer Yann2ORCID, Conti Anita1, Pozza Edoardo Dalla3, Vigato Enrico3, Malatesta Manuela1ORCID, Calderan Laura1ORCID
Affiliation:
1. Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy 2. PEPITE EA4267, Université Franche-Comté, 25030 Besançon, France 3. Department of Plastic and Reconstructive Surgery, Verona University Hospital (A.O.U.I. Verona), 37126 Verona, Italy
Abstract
Skin is a major administration route for drugs, and all transdermal formulations must be tested for their capability to overcome the cutaneous barrier. Therefore, developing highly reliable skin models is crucial for preclinical studies. The current in vitro models are unable to replicate the living skin in all its complexity; thus, to date, excised human skin is considered the gold standard for in vitro permeation studies. However, skin explants have a limited life span. In an attempt to overcome this problem, we used an innovative bioreactor that allowed us to achieve good structural and functional preservation in vitro of explanted human skin for up to 72 h. This device was then used to set up an in vitro inflammatory model by applying two distinct agents mimicking either exogenous or endogenous stimuli: i.e., dithranol, inducing the contact dermatitis phenotype, and the substance P, mimicking neurogenic inflammation. Our in vitro system proved to reproduce inflammatory events observed in vivo, such as vasodilation, increased number of macrophages and mast cells, and increased cytokine secretion. This bioreactor-based system may therefore be suitably and reliably used to simulate in vitro human skin inflammation and may be foreseen as a promising tool to test the efficacy of drugs and cosmetics.
Funder
French Region Bourgogne Franche Comté
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference69 articles.
1. Skin biology;Hwa;Dermatol. Ther.,2011 2. Overcoming the skin permeation barrier: Challenges and opportunities;Vitorino;Curr. Pharm. Des.,2015 3. Gorzelanny, C., Mess, C., Schneider, S.W., Huck, V., and Brandner, J.M. (2020). Skin Barriers in Dermal Drug Delivery: Which Barriers Have to Be Overcome and How Can We Measure Them?. Pharmaceutics, 12. 4. Dragicevic, N., and Maibach, H.I. (2015). Percutaneous Penetration Enhancers: Chemical Methods in Penetration Enhancement, Springer. 5. Russell, W.M.S., and Burch, R.L. (1959). The Principles of Humane Experimental Technique, Methuen & Co.. [1st ed.].
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|