Ximenia americana L.: Chemical Characterization and Gastroprotective Effect
-
Published:2023-04-30
Issue:2
Volume:4
Page:141-158
-
ISSN:2673-4532
-
Container-title:Analytica
-
language:en
-
Short-container-title:Analytica
Author:
Pessoa Renata Torres1ORCID, Alcântara Isabel Sousa1, da Silva Lucas Yure Santos1, da Costa Roger Henrique Souza1, Silva Tarcísio Mendes1, de Morais Oliveira-Tintino Cícera Datiane2, Ramos Andreza Guedes Barbosa1, de Oliveira Maria Rayane Correia13ORCID, Martins Anita Oliveira Brito Pereira Bezerra1, de Lacerda Bruna Caroline Gonçalves Vasconcelos4, de Andrade Edlane Martins4ORCID, Ribeiro-Filho Jaime5ORCID, Gonçalves Lima Clara Mariana6ORCID, Coutinho Henrique Douglas Melo2ORCID, Menezes Irwin Rose Alencar de1ORCID
Affiliation:
1. Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Crato 63105-000, CE, Brazil 2. Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Crato 63105-000, CE, Brazil 3. Graduate Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), State University of Ceará (UECE), Av. Dr. Silas Munguba, 1700, Fortaleza 60741-000, CE, Brazil 4. CECAPE College, Av. Padre Cícero, 3917, Juazeiro do Norte 63024-015, CE, Brazil 5. Department of Biotechnology, Oswaldo Cruz Foundation, Fiocruz Ceará, Eusébio, Fortaleza 60180-190, CE, Brazil 6. Department of Food Science, Federal University of Lavras, Lavras 37203-202, MG, Brazil
Abstract
Ximenia americana L., popularly known in Brazil as “ameixa do-mato, ameixa-brava, and ameixa-do-sertão,” is widely used in folk medicine to treat several intestinal disorders. The present study assessed the potential mechanisms of action underlying the gastroprotective activity of the hydroethanolic extract of Ximenia americana L. (EHXA) stem bark. The acute toxicity of EHXA was estimated, and later, the gastroprotective effect in mice was assessed through acute models of gastric lesions induced by acidified or absolute ethanol and indomethacin, where the following mechanisms were pharmacologically analyzed: the involvement of prostaglandins (PG), histamine (H2) receptors, ATP-dependent potassium channels, sulfhydryl groups (SH), α2 adrenergic receptors, nitric oxide (NO), myeloperoxidase (MPO), gastric mucus production, and acetylcholine-mediated intestinal motility. Regarding toxicity, EHXA did not cause deaths or signs of toxicity (LD50 greater than or equal to 2000 mg/kg/p.o.). When the gastroprotective effect was assessed, EHXA (50, 100, and 200 mg/kg/p.o.) reduced the rate of lesions induced by acidified ethanol by 65.63; 53.66, and 58.02% in absolute ethanol at 88.91, 78.82, and 74.68%, respectively, when compared to the negative control group. In the indomethacin-induced gastric injury model, the reductions were 84.69, 55.99, 55.99, and 42.50%, respectively. The study revealed that EHXA might stimulate mucus production and reduce intestinal motility through SH groups, NO production, and activation of α2 adrenergic receptors. The results indicated that EHXA had significant gastroprotective activity in the evaluated models. However, further investigation is required to elucidate the cellular and molecular events underlying the action of EHXA components and to correlate them with the modulation of the signaling pathways, as demonstrated by the current pharmacological approach. Therefore, the results demonstrated in the present study, as well as previously reported findings, support the recommendation of using this species in traditional communities in Brazil.
Funder
Brazilian agencies CAPES Nacional Institute of Science and Technology—Ethnobiology, Bioprospecting and Nature Conservation/CNPq/FACEPE
Reference88 articles.
1. De Filippis, A., Ullah, H., Baldi, A., Dacrema, M., Esposito, C., Garzarella, E.U., Santarcangelo, C., Tantipongpiradet, A., and Daglia, M. (2020). Gastrointestinal Disorders and Metabolic Syndrome: Dysbiosis as a Key Link and Common Bioactive Dietary Components Useful for Their Treatment. Int. J. Mol. Sci., 21. 2. Observational Survey of NSAID-Related Upper Gastrointestinal Adverse Events in Belgium;Belaiche;Acta Gastroenterol. Belg.,2002 3. The Association of Helicobacter Pylori Infection and Nonsteroidal Anti-Inflammatory Drugs in Peptic Ulcer Disease;Can. J. Gastroenterol.,2006 4. Abarema Cochliacarpos: Gastroprotective and Ulcer-Healing Activities;Vilegas;J. Ethnopharmacol.,2010 5. Antiulcerogenic Activity of Extract, Fractions, and Some Compounds Obtained from Polygala cyparissias St. Hillaire & Moquin (Polygalaceae);Klein;Naunyn-Schmiedeberg’s Arch. Pharmacol.,2010
|
|