Affiliation:
1. Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia
2. University Medical Service Center, Building 70, King Abdulaziz University, Jeddah 21589, Saudi Arabia
3. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11725, Egypt
Abstract
Lawsonia inermis, known as henna, has traditionally been utilized in cosmetics and folk medicine because of their valuable health effects. A lack of information about the processes that increase or decrease release, as well as the biological activities of constituents of natural origin, is an important pharmacological problem. This investigation evaluates the influence of moist heat on the flavonoid and phenolic contents of henna powder and their biological activities. HPLC analysis reflected the existence of 20 and 19 compounds of flavonoids and phenolics in the extract of unpre-treated henna by moist heat (UPMH) and pre-treated henna by moist heat (PMH). Several compounds such as chlorogenic acid, ellagic acid, rutin, rosmarinic acid, kaempferol, and pyrocatechol occurred with high concentrations of 57,017.33, 25,821.09, 15,059.88, 6345.08, 1248.42, and 819.19 µg/mL UPMH while occurred with low concentrations of 44,286.51, 17,914.26, 3809.85, 5760.05, 49.01, and 0.0 µg/mL, respectively in PMH. C. albicans, C. tropicalis, and G. candidum were more affected by UPMH with inhibition zones of 30.17 ± 0.29, 27 ± 0.5, and 29 ± 1.5 mm than PMH with inhibition zones of 29 ± 0.5, 25.33 ± 0.58, and 24.17 ± 0.29 mm, respectively. UPMH henna exhibited less MIC and MFC against the tested yeasts than PMH. Moreover, UPMH henna showed good wound healing, where the rat of migration, wound closure %, and area difference % were 14.806 um, 74.938 um2, and 710.667% compared with PMH henna 11.360 um, 59.083 um2, 545.333%, respectively. Antioxidant activity of UPMH and PMH henna. Promising antioxidant activity was recorded for both UPMH or PMH henna with IC50 5.46 µg/mL and 7.46 µg/mL, respectively. The docking interaction of chlorogenic acid and ellagic acid with the crystal structures of G. candidum (4ZZT) and C. albicans (4YDE) was examined. The biological screening demonstrated that the compounds had favorable docking results with particular proteins. Chlorogenic acid had robust behavior in the G. candidum (4ZZT) active pocket and displayed a docking score of −7.84379 Kcal/mol, higher than ellagic acid’s −6.18615 Kcal/mol.
Funder
Deanship of Scientific Research at Imam Mohammad Ibn Saud Islamic University
Subject
Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics
Reference38 articles.
1. Preliminary phytochemical screening and antibacterial activity of Lawsonia inermis Linn (Henna) leaf extracts against reference bacterial strains and clinically important AMPC beta-lactamases producing Proteus mirabilis;Jothiprakasam;Int. J. Pharm. Pharm. Sci.,2013
2. Antifungal activity of the Algerian Lawsonia inermis (henna);Rahmoun;Pharm. Biol.,2013
3. Antifungal activity of Lawsonia inermis L. (Henna) against clinical Candida isolates;Erzincan Univ. J. Sci. Technol.,2017
4. Phytochemical and Pharmacological Activities of Natural Dye Plant, Lawsonia inermis L. (Henna);Supian;J. Young Pharm.,2023
5. Clinical and microbiological diagnosis of oral candidiasis;J. Clin. Exp. Dent.,2013