New Pyrroline Isolated from Antarctic Krill-Derived Actinomycetes Nocardiopsis sp. LX-1 Combining with Molecular Networking
-
Published:2023-02-15
Issue:2
Volume:21
Page:127
-
ISSN:1660-3397
-
Container-title:Marine Drugs
-
language:en
-
Short-container-title:Marine Drugs
Author:
Shi Ting12ORCID, Li Yan-Jing1, Wang Ze-Min2, Wang Yi-Fei1, Wang Bo1ORCID, Shi Da-Yong2ORCID
Affiliation:
1. College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao 266590, China 2. State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China
Abstract
Antarctic krill (Euphausia superba) of the Euphausiidae family comprise one of the largest biomasses in the world and play a key role in the Antarctic marine ecosystem. However, the study of E. superba-derived microbes and their secondary metabolites has been limited. Chemical investigation of the secondary metabolites of the actinomycetes Nocardiopsis sp. LX-1 (in the family of Nocardiopsaceae), isolated from E. superba, combined with molecular networking, led to the identification of 16 compounds a–p (purple nodes in the molecular network) and the isolation of one new pyrroline, nocarpyrroline A (1), along with 11 known compounds 2–12. The structure of the new compound 1 was elucidated by extensive spectroscopic investigation. Compound 2 exhibited broad-spectrum antibacterial activities against A. hydrophila, D. chrysanthemi, C. terrigena, X. citri pv. malvacearum and antifungal activity against C. albicans in a conventional broth dilution assay. The positive control was ciprofloxacin with the MIC values of <0.024 µM, 0.39 µM, 0.39 µM, 0.39 µM, and 0.20 µM, respectively. Compound 1 and compounds 7, 10, and 11 displayed antifungal activities against F. fujikuroi and D. citri, respectively, in modified agar diffusion test. Prochloraz was used as positive control and showed the inhibition zone radius of 17 mm and 15 mm against F. fujikuroi and D. citri, respectively. All the annotated compounds a–p by molecular networking were first discovered from the genus Nocardiopsis. Nocarpyrroline A (1) features an unprecedented 4,5-dihydro-pyrrole-2-carbonitrile substructure, and it is the first pyrroline isolated from the genus Nocardiopsis. This study further demonstrated the guiding significance of molecular networking in the research of microbial secondary metabolites.
Funder
Shandong Provincial Natural Science Foundation National Natural Science Foundation of China Talent Support Program of Shandong University of Science and Technology Top-notch Young Professionals
Subject
Drug Discovery,Pharmacology, Toxicology and Pharmaceutics (miscellaneous),Pharmaceutical Science
Reference46 articles.
1. Sesquiterpenoids from the Antarctic fungus Pseudogymnoascus sp. HSX2#-11;Shi;Front. Microbiol.,2021 2. Colletti, A., Cravotto, G., Citi, V., Martelli, A., Testai, L., and Cicero, A.F. (2021). Advances in technologies for highly active omega-3 fatty acids from krill oil: Clinical applications. Mar. Drugs, 19. 3. Continuous moulting by Antarctic krill drives major pulses of carbon export in the north Scotia Sea, Southern Ocean;Manno;Nat. Commun.,2020 4. Huang, Y., Bian, C., Liu, Z., Wang, L., Xue, C., Huang, H., Yi, Y., You, X., Song, W., and Mao, X. (2020). The first genome survey of the Antarctic Krill (Euphausia superba) provides a valuable genetic resource for polar biomedical research. Mar. Drugs, 18. 5. The fishery for Antarctic krill—Recent developments;Nicol;Fish Fish.,2012
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|