Identification of Putative Quantitative Trait Loci for Improved Seed Oil Quality in Peanuts

Author:

Hu Pengju1,Zhang Jianan1,Song Yahui1,Zhao Xing1,Jin Xinxin1,Su Qiao1,Yang Yongqing1ORCID,Wang Jin1

Affiliation:

1. The Key Laboratory of Crop Genetics and Breeding of Hebei, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China

Abstract

Improving seed oil quality in peanut (Arachis hypogaea) has long been an aim of breeding programs worldwide. The genetic resources to achieve this goal are limited. We used an advanced recombinant inbred line (RIL) population derived from JH5 × KX01-6 to explore quantitative trait loci (QTL) affecting peanut oil quality and their additive effects, epistatic effects, and QTL × environment interactions. Gas chromatography (GC) analysis suggested seven fatty acids components were obviously detected in both parents and analyzed in a follow-up QTL analysis. The major components, palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2), exhibited considerable phenotypic variation and fit the two major gene and minor gene mixed-inheritance model. Seventeen QTL explained 2.57–38.72% of the phenotypic variation in these major components, with LOD values of 4.12–37.56 in six environments, and thirty-five QTL explained 0.94–32.21% of the phenotypic variation, with LOD values of 5.99–150.38 in multiple environments. Sixteen of these QTL were detected in both individual and multiple environments. Among these, qFA_08_1 was a novel QTL with stable, valuable and major effect. Two other major-effect QTL, qFA_09_2 and qFA_19_3, share the same physical position as FAD2A and FAD2B, respectively. Eleven stable epistatic QTL involving nine loci explained 1.30–34.97% of the phenotypic variation, with epistatic effects ranging from 0.09 to 6.13. These QTL could be valuable for breeding varieties with improved oil quality.

Funder

Science and Technology Innovation Team of modern peanut seed industry

modern agricultural industrial technology system of Hebei Province

earmarked fund for CARS-13

HAAFS Science and Technology Innovation Special Project

Basic Research Funds of Hebei Academy of Agriculture and Forestry Sciences

Talents construction project of science and technology innovation, Hebei Academy of Agriculture and Forestry Sciences

Publisher

MDPI AG

Reference31 articles.

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