Integrated Transcriptomics and Metabolomics Analysis of Two Maize Hybrids (ZD309 and XY335) under Heat Stress at the Flowering Stage
Author:
Zhao Pu1, Sun Lei1, Zhang Siqi12, Jiao Bo1, Wang Jiao1, Ma Chunhong1
Affiliation:
1. Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Science/Hebei Key Laboratory of Plant Genetic Engineering, Shijiazhuang 050051, China 2. College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
Abstract
High temperature around flowering has a serious impact on the growth and development of maize. However, few maize genes related to flowering under heat stress have been confirmed, and the regulatory mechanism is unclear. To reveal the molecular mechanism of heat tolerance in maize, two maize hybrids, ZD309 and XY335, with different heat resistance, were selected to perform transcriptome and metabolomics analysis at the flowering stage under heat stress. In ZD309, 314 up-regulated and 463 down-regulated differentially expressed genes (DEGs) were detected, while 168 up-regulated and 119 down-regulated DEGs were identified in XY335. By comparing the differential gene expression patterns of ZD309 and XY335, we found the “frontloaded” genes which were less up-regulated in heat-tolerant maize during high temperature stress. They included heat tolerance genes, which may react faster at the protein level to provide resilience to instantaneous heat stress. A total of 1062 metabolites were identified via metabolomics analysis. Lipids, saccharides, and flavonoids were found to be differentially expressed under heat stress, indicating these metabolites’ response to high temperature. Our study will contribute to the identification of heat tolerance genes in maize, therefore contributing to the breeding of heat-tolerant maize varieties.
Funder
Hebei Natural Science Foundation Hebei Province Foundation for the Introduce Returned Overseas Chinese Scholar
Subject
Genetics (clinical),Genetics
Reference50 articles.
1. Liu, J., Zhang, L., Huang, L., Yang, T., Ma, J., Yu, T., Zhu, W., Zhang, Z., and Tang, J. (2022). Uncovering the Gene Regulatory Network of Maize Hybrid ZD309 under Heat Stress by Transcriptomic and Metabolomic Analysis. Plants, 11. 2. Djalovic, I., Kundu, S., Bahuguna, R.N., Pareek, A., Raza, A., Singla-Pareek, S.L., Prasad, P.V.V., and Varshney, R.K. (2023). Maize and heat stress: Physiological, genetic, and molecular insights. Plant Genome, e20378. 3. Temperature increase reduces global yields of major crops in four independent estimates;Zhao;Proc. Natl. Acad. Sci. USA,2017 4. Physiological and biochemical responses of hybrid maize (Zea mays L.) varieties grown under heat stress conditions;Tas;PeerJ,2022 5. Field crops and the fear of heat stress—Opportunities, challenges and future directions;Prasad;Field Crops Res.,2017
|
|