Analysis of the Molecular Mechanism of Energy Metabolism in the Sex Differentiation of Chickens Based on Transcriptome Sequencing-Reference-Cited by-同舟云学术

Analysis of the Molecular Mechanism of Energy Metabolism in the Sex Differentiation of Chickens Based on Transcriptome Sequencing

Published:2024-08-06 Issue:8 Volume:15 Page:1035
ISSN:2073-4425
Container-title:Genes
language:en
Short-container-title:Genes

Author:

Zhao Ziduo¹²,Zhao Zongyi¹²,Cheng Fufu¹²,Wang Zhe¹²,Geng Qingqing¹²,Wang Yingjie³,Niu Yingjie¹²^ORCID,Zuo Qisheng¹²,Zhang Yani¹²^ORCID

Affiliation:

1. Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

2. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

3. College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China

Abstract

The determination of sex in mammals is established and controlled by various complex mechanisms. In contrast, sex control in poultry remains an unresolved issue. In this study, RNA-sequencing was conducted for male gonads and ovarian tissues in chicken embryos of up to 18.5 days to identify metabolic factors influencing male and female sex differentiation, as well as gonadal development. Our results reveal that PKM2, a critical glycolysis-related protein, plays a significant role in chicken sex differentiation via PPARG, a crucial hormone gene. We propose that our discoveries bolster the notion that glycolysis and oxidative phosphorylation function as antecedent contributors to sexual phenotypic development and preservation.

Funder

National Key R&D Program of China

National Natural Science Foundation of China

Jiangsu Province Graduate Practice Innovation Program

Jiangsu Province Research and Practice Innovation Program for Graduate Students

Publisher

MDPI AG

Link

https://www.mdpi.com/2073-4425/15/8/1035/pdf

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