Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos
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Published:2023-04-07
Issue:4
Volume:12
Page:897
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Zhang Peipei1, Yang Baigao1, Xu Xi1, Zhang Hang1, Feng Xiaoyi1, Hao Haisheng1, Du Weihua1ORCID, Zhu Huabin1, Li Shujing2, Yu Wenli2, Khan Adnan3ORCID, Umer Saqib4ORCID, Zhao Xueming1ORCID
Affiliation:
1. Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China 2. Shijiazhuang Tianquan Elite Dairy Ltd., Shijiazhuang 050200, China 3. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China 4. Department of Theriogenology, University of Agriculture, Faisalabad 38000, Punjab, Pakistan
Abstract
Oocyte maturation is a critical step in the completion of female gametogenesis in the ovary; thus, for subsequent fertilization and embryogenesis. Vitrification of embryo also has been shown to be closely associated with oocyte maturation. To improve the quality and developmental potential of bovine oocytes derived from in vitro maturation (IVM), Pre-IVM with C-type natriuretic peptide (CNP), melatonin (MT) and in combination, IGF1, FGF2, LIF (FLI) were supplemented in the IVM medium. In this current study, we cultured bovine oocytes in Pre-IVM with CNP for 6 h before transferring them to the IVM medium supplemented with MT and FLI. The developmental potential of bovine oocytes was then investigated by measuring the reactive oxygen species (ROS), the intracellular glutathione (GSH) and ATP levels, the transzonal projections (TZP), the mitochondrial membrane potential (ΔΨm), cacline-AM, and the expression of related genes (cumulus cells (CCs), oocytes, blastocysts). The results revealed that oocytes treated with a combination of CNP, MT, and FLI had dramatically improved the percentage of oocytes developed to blastocyst, ATP content, GSH levels, TZP intensity, the ΔΨm, cacline-AM fluorescence intensity, and considerably reduced ROS levels of oocytes. Furthermore, the survival rate and the hatched rate after vitrification of the CNP+MT+FLI group were significantly higher than those other groups. Thus, we speculated that CNP+MT+FLI increases the IVM of bovine oocytes. In conclusion, our findings deepen our understanding and provide new perspectives on targeting the combination of CNP, MT and FLI to enhance the quality and developmental potential of bovine oocytes.
Funder
National Key R&D Program of China National Natural Science Foundation of China Natural Science Foundation of Beijing National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province Key Research and Development Program of Hebei Province Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
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