Anti-Inflammatory and Anti-Oxidative Effects of Polysaccharides Extracted from Unripe Carica papaya L. Fruit

Author:

Lin Ting-Yun1,Wu Yun-Ting1,Chang Hui-Ju2,Huang Chun-Chen1,Cheng Kuan-Chen3456ORCID,Hsu Hsien-Yi78,Hsieh Chang-Wei16ORCID

Affiliation:

1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung City 402202, Taiwan

2. Department of Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Executive Yuan, Taichung City 426017, Taiwan

3. Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan

4. Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan

5. Department of Optometry, Asia University, Taichung City 413305, Taiwan

6. Department of Medical Research, China Medical University Hospital, Taichung City 404333, Taiwan

7. Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China

8. Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong 999077, China

Abstract

This study evaluated the antioxidative and anti-inflammatory activities of polysaccharides extracted from unripe Carica papaya L. (papaya) fruit. Three papaya polysaccharide (PP) fractions, namely PP-1, PP-2, and PP-3, with molecular weights of 2252, 2448, and 3741 kDa, containing abundant xylose, galacturonic acid, and mannose constituents, respectively, were obtained using diethylaminoethyl–Sepharose™ anion exchange chromatography. The antioxidant capacity of the PPs, hydroxyl radical scavenging assay, ferrous ion-chelating assay, and reducing power assay revealed that the PP-3 fraction had the highest antioxidant activity, with an EC50 (the concentration for 50% of the maximal effect) of 0.96 mg/mL, EC50 of 0.10 mg/mL, and Abs700 nm of 1.581 for the hydroxyl radical scavenging assay, ferrous ion-chelating assay, and reducing power assay, respectively. In addition, PP-3 significantly decreased reactive oxygen species production by 45.3%, NF-κB activation by 32.0%, and tumor necrosis factor-alpha and interleukin-6 generation by 33.5% and 34.4%, respectively, in H2O2-induced human epidermal keratinocytes. PP-3 exerts potent antioxidative and anti-inflammatory effects; thus, it is a potential biofunctional ingredient in the cosmetic industry.

Funder

Council of Agriculture, Executive Yuan, Taiwan

Publisher

MDPI AG

Subject

Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology

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