Hydrogen Sulfide Downregulates Oncostatin M Expression via PI3K/Akt/NF-κB Signaling Processes in Neutrophil-like Differentiated HL-60 Cells
-
Published:2023-02-08
Issue:2
Volume:12
Page:417
-
ISSN:2076-3921
-
Container-title:Antioxidants
-
language:en
-
Short-container-title:Antioxidants
Author:
Han Na-Ra12, Ko Seong-Gyu23ORCID, Park Hi-Joon4, Moon Phil-Dong5
Affiliation:
1. College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 2. Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 3. Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 4. Department of Anatomy & Information Sciences, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 5. Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea
Abstract
The cytokine oncostatin M (OSM) is regarded as a critical mediator in various inflammatory responses. While the gaseous signaling molecule hydrogen sulfide (H2S) plays a role in a variety of pathophysiological conditions, such as hypertension, inflammatory pain, osteoarthritis, ischemic stroke, oxidative stress, retinal degeneration, and inflammatory responses, the underlying mechanism of H2S action on OSM expression in neutrophils needs to be clarified. In this work, we studied how H2S reduces OSM expression in neutrophil-like differentiated (d)HL-60 cells. To evaluate the effects of H2S, sodium hydrosulfide (NaHS, a donor that produces H2S), ELISA, real-time PCR (qPCR), immunoblotting, and immunofluorescence staining were utilized. Although exposure to granulocyte–macrophage colony-stimulating factor (GM-CSF) resulted in upregulated levels of production and mRNA expression of OSM, these upregulated levels were reduced by pretreatment with NaHS in dHL-60 cells. Similarly, the same pretreatment lowered phosphorylated levels of phosphatidylinositol 3-kinase, Akt, and nuclear factor-kB that had been elevated by stimulation with GM-CSF. Overall, our results indicated that H2S could be a therapeutic agent for inflammatory disorders via suppression of OSM.
Funder
National Research Foundation of Korea (NRF) grant funded by the Korea government
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference67 articles.
1. Hsu, C.N., Hou, C.Y., Chang-Chien, G.P., Lin, S., Yang, H.W., and Tain, Y.L. (2022). Sodium Thiosulfate Improves Hypertension in Rats with Adenine-Induced Chronic Kidney Disease. Antioxidants, 11. 2. Piñeiro-Ramil, M., Burguera, E.F., Hermida-Gómez, T., Caramés, B., Oreiro-Villar, N., Meijide-Faílde, R., Blanco, F.J., and Vaamonde-García, C. (2022). Reduced Levels of H2S in Diabetes-Associated Osteoarthritis Are Linked to Hyperglycaemia, Nrf-2/HO-1 Signalling Downregulation and Chondrocyte Dysfunction. Antioxidants, 11. 3. Porta, A., Rodríguez, L., Bai, X., Batallé, G., Roch, G., Pouso-Vázquez, E., Balboni, G., and Pol, O. (2021). Hydrogen Sulfide Inhibits Inflammatory Pain and Enhances the Analgesic Properties of Delta Opioid Receptors. Antioxidants, 10. 4. Therapeutic applications of hydrogen sulfide and novel donors for cerebral ischemic stroke: A narrative review;Ding;Med. Gas Res.,2023 5. Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal;Zhu;Redox Rep.,2022
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|