Pressurized Liquid (PLE) Truffle Extracts Have Inhibitory Activity on Key Enzymes Related to Type 2 Diabetes (α-Glucosidase and α-Amylase)-Reference-Cited by-全球学者库

Pressurized Liquid (PLE) Truffle Extracts Have Inhibitory Activity on Key Enzymes Related to Type 2 Diabetes (α-Glucosidase and α-Amylase)

Published:2023-07-17 Issue:14 Volume:12 Page:2724
ISSN:2304-8158
Container-title:Foods
language:en
Short-container-title:Foods

Author:

Tejedor-Calvo Eva¹²³^ORCID,Morales Diego¹⁴^ORCID,Morillo Laura¹^ORCID,Vega Laura¹,Caro Mercedes³,Smiderle Fhernanda Ribeiro⁵⁶,Iacomini Marcello⁷,Marco Pedro²^ORCID,Soler-Rivas Cristina¹

Affiliation:

1. Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain

2. Department of Forest Resources, Agrifood Research and Technology Centre of Aragon (CITA), Agrifood Institute of Aragón—IA2 (CITA-Zaragoza University), Av. Montañana 930, 50059 Zaragoza, Spain

3. AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, Spain

4. Departmental Section of Galenic Pharmacy and Food Technology, Veterinary Faculty, Complutense University of Madrid, 28040 Madrid, Spain

5. Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil

6. Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil

7. Department of Biochemistry and Molecular Biology, Federal University of Parana, Curitiba 81531-980, PR, Brazil

Abstract

An optimized PLE method was applied to several truffle species using three different solvent mixtures to obtain bioactive enriched fractions. The pressurized water extracts contained mainly (1 → 3),(1 → 6)-β-D-glucans, chitins, and heteropolymers with galactose and mannose in their structures. The ethanol extracts included fatty acids and fungal sterols and others such as brassicasterol and stigmasterol, depending on the species. They also showed a different fatty acid lipid profile depending on the solvent utilized and species considered. Ethanol:water extracts showed interesting lipids and many phenolic compounds; however, no synergic extraction of compounds was noticed. Some of the truffle extracts were able to inhibit enzymes related to type 2 diabetes; pressurized water extracts mainly inhibited the α-amylase enzyme, while ethanolic extracts were more able to inhibit α-glucosidase. Tuber brumale var. moschatum and T. aestivum var. uncinatum extracts showed an IC50 of 29.22 mg/mL towards α-amylase and 7.93 mg/mL towards α-glucosidase. Thus, use of the PLE method allows o bioactive enriched fractions to be obtained from truffles with antidiabetic properties.

Funder

Ibercaja-CAI Estancias de Investigación

European Union

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Link

https://www.mdpi.com/2304-8158/12/14/2724/pdf

Reference49 articles.

1. A Review of the Anti-inflammatory Properties of Antidiabetic Agents Providing Protective Effects against Vascular Complications in Diabetes;Yaribeygi;J. Cell Physiol.,2019

2. Impact of Postprandial Glycaemia on Health and Prevention of Disease;Blaak;Obes. Rev.,2012

3. Antidiabetic Activity of Ergosterol from Pleurotus Ostreatus in KK-A y Mice with Spontaneous Type 2 Diabetes Mellitus;Xiong;Mol. Nutr. Food Res.,2018

4. An Insight into Antidiabetic Properties of Six Medicinal and Edible Mushrooms: Inhibition of α-Amylase and α-Glucosidase Linked to Type-2 Diabetes;Stojkovic;S. Afr. J. Bot.,2019

5. Oliach, D., Vidale, E., Brenko, A., Marois, O., Andrighetto, N., Stara, K., Mart, J., Arag, D., Colinas, C., and Bonet, A. (2021). Truffle Market Evolution: An Application of the Delphi Method. Forests, 12.