Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats
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Published:2023-05-25
Issue:11
Volume:24
Page:9230
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Sugahara Shinpei1, Haga Hiroaki1ORCID, Ikeda Chisaki1, Makino Naohiko2ORCID, Matsuda Akiko1, Kakizaki Yasuharu1, Hoshikawa Kyoko1, Katsumi Tomohiro1, Ishizawa Tetsuya1, Kobayashi Toshikazu1, Maki Keita1, Suzuki Fumiya1, Murakami Ryoko3, Sato Hidenori3, Ueno Yoshiyuki1ORCID
Affiliation:
1. Department of Gastroenterology, Faculty of Medicine, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-8595, Japan 2. Yamagata University Health Administration Center, 1-4-12 Kojirakawa-Machi, Yamagata 990-8560, Japan 3. Genomic Information Analysis Unit, Department of Genomic Cohort Research, Faculty of Medicine, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-8595, Japan
Abstract
Although liver regeneration has been extensively studied, the effects of bile-derived extracellular vesicles (bile EVs) on hepatocytes has not been elucidated. We examined the influence of bile EVs, collected from a rat model of 70% partial hepatectomy (PH), on hepatocytes. We produced bile-duct-cannulated rats. Bile was collected over time through an extracorporeal bile duct cannulation tube. Bile EVs were extracted via size exclusion chromatography. The number of EVs released into the bile per liver weight 12 h after PH significantly increased. Bile EVs collected 12 and 24 h post-PH, and after sham surgery (PH12-EVs, PH24-EVs, sham-EVs) were added to the rat hepatocyte cell line, and 24 h later, RNA was extracted and transcriptome analysis performed. The analysis revealed that more upregulated/downregulated genes were observed in the group with PH24-EVs. Moreover, the gene ontology (GO) analysis focusing on the cell cycle revealed an upregulation of 28 types of genes in the PH-24 group, including genes that promote cell cycle progression, compared to the sham group. PH24-EVs induced hepatocyte proliferation in a dose-dependent manner in vitro, whereas sham-Evs showed no significant difference compared to the controls. This study revealed that post-PH bile Evs promote the proliferation of the hepatocytes, and genes promoting cell cycles are upregulated in hepatocytes.
Funder
Japan Society for the Promotion of Science (JSPS) Kakenhi
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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