Abstract
Background: The variability of prion protein gene (PRNP) codons and the frequency of alleles (K222, D146, and S146) that appear to confer genetic resistance to classical scrapie are still unknown in several goat populations/breeds prevalent in Romania. This work aims to assess the genetic polymorphism at 15 PRNP codons in Romanian goat populations to inform the development of goat breeding programs for scrapie resistance. Methods: Whole blood and hair follicles from Carpathian (50), French Alpine (53), and Banat’s White (53) breed goats were sampled to extract genomic DNA for genetic analyses and Sanger sequencing. In the targeted goat groups, one classical scrapie-positive Banat’s White goat was included. Results: The codons without polymorphisms were G37G, W102W, N146N, R151R, S173S, and I218I. The following non-synonymous polymorphisms of PRNP were recorded: P110P, P110S, P110T, T110T, G127G, G127S, I142I, I142M, T142I, H143H, P143P, R143R, R154R, H154R, P168P, Q168Q, Q211Q, Q211R, Q222Q, H222Q, K222K, S240S, P240P, P240S, and S240P. Conclusions: PRNP polymorphism was recorded in 60% (9/15) of codons. The scrapie-positive Banat’s White goat had G37G, W102W, T110T, G127G, I142I, H143H, N146N, R151R, R154R, P168P, S173S, R211R, I218I, Q222Q, and S240S. The K222 allele had a frequency of 6% (3/50) in Carpathian, 9.43% (5/53) in Banat’s White, and 15.09% (8/53) in French Alpine. Therefore, the polymorphisms detected in this sample of Romanian goat breeds are too rare to design a breeding program at the current time.
Funder
University of Agronomic Sciences and Veterinary Medicine of Bucharest
Subject
Genetics (clinical),Genetics
Reference58 articles.
1. Molecular biology and genetics of prion diseases;Prusiner;Philos. Trans. R. Soc. Lond. B Biol. Sci.,1994
2. Spatio-temporal and risk factor analysis of alleles related to Scrapie resistance in sheep in Great Britain before, during and after a national breeding program
3. Consolidated Text: Regulation (EC) No 999/2001 of the European Parliament and of the Council of 22 May 2001 Laying down Rules for the Prevention. Control and Eradication of Certain Transmissible Spongiform Encephalopathies
http://eur-lex.europa.eu/legal-content/EN/TXT/?qid=1519030609850&uri=CELEX:02001R0999-20170701
4. Advancing Prion Science: Guidance for the National Prion Research Program;Institute of Medicine (US) Committee on Transmissible Spongiform Encephalopathies: Assessment of Relevant Science,2003
5. Risk management of the transmissible spongiform encephalopathies in North America